| Baculovirus per os infectivity factors form a complex on the surface of occlusion-derived virus | |
| Peng, Ke1,2; van Oers, Monique M.1; Hu, Zhihong2; van Lent, Jan W. M.1; Vlak, Just M.1 | |
| 刊名 | Journal of virology
 |
| 2010-09-15 | |
| 卷号 | 84期号:18页码:9497-9504 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.00812-10 |
| 通讯作者 | Van oers, monique m.(monique.vanoers@wur.nl) |
| 英文摘要 | Five highly conserved per os infectivity factors, pif1, pif2, pif3, pif4, and p74, have been reported to be essential for oral infectivity of baculovirus occlusion-derived virus (odv) in insect larvae. three of these proteins, p74, pif1, and pif2, were thought to function in virus binding to insect midgut cells. in this paper evidence is provided that pif1, pif2, and pif3 form a stable complex on the surface of odv particles of the baculovirus autographa californica multinucleocapsid nucleopolyhedrovirus (acmnpv). the complex could withstand 2% sds-5% beta-mercaptoethanol with heating at 50 degrees c for 5 min. the complex was not formed when any of the genes for pif1, pif2, or pif3 was deleted, while reinsertion of these genes into acmnpv restored the complex. coimmunoprecipitation analysis independently confirmed the interactions of the three pif proteins and revealed in addition that p74 is also associated with this complex. however, deletion of the p74 gene did not affect formation of the pif1-pif2-pif3 complex. electron microscopy analysis showed that pif1 and pif2 are localized on the surface of the odv with a scattered distribution. this distribution did not change for pif1 or pif2 when the gene for pif2 or pif1 protein was deleted. we propose that pif1, pif2, pif3, and p74 form an evolutionarily conserved complex on the odv surface, which has an essential function in the initial stages of baculovirus oral infection. |
| WOS关键词 | PROTEIN-PROTEIN INTERACTIONS ; HELIOTHIS-VIRESCENS LARVAE ; IN-VIVO ; NUCLEOPOLYHEDROVIRUS ; ENTRY ; ENVELOPE ; FUSION ; P74 ; IDENTIFICATION ; BINDING |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | AMER SOC MICROBIOLOGY |
| WOS记录号 | WOS:000281110500050 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375730 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | van Oers, Monique M. |
| 作者单位 | 1.Wageningen Univ, Virol Lab, NL-6708 PB Wageningen, Netherlands 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Peng, Ke,van Oers, Monique M.,Hu, Zhihong,et al. Baculovirus per os infectivity factors form a complex on the surface of occlusion-derived virus[J]. Journal of virology,2010,84(18):9497-9504. |
| APA | Peng, Ke,van Oers, Monique M.,Hu, Zhihong,van Lent, Jan W. M.,&Vlak, Just M..(2010).Baculovirus per os infectivity factors form a complex on the surface of occlusion-derived virus.Journal of virology,84(18),9497-9504. |
| MLA | Peng, Ke,et al."Baculovirus per os infectivity factors form a complex on the surface of occlusion-derived virus".Journal of virology 84.18(2010):9497-9504. |
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