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	The vici syndrome protein epg5 is a rab7 effector that determines the fusion specificity of autophagosomes with late endosomes/lysosomes
	Wang, Zheng1; Miao, Guangyan1,2; Xue, Xue1; Guo, Xiangyang3; Yuan, Chongzhen1; Wang, Zhaoyu1; Zhang, Gangming1; Chen, Yingyu2; Feng, Du4; Hu, Junjie1
刊名	Molecular cell
	2016-09-01
卷号	63期号:5页码:781-795
ISSN号	1097-2765
DOI	10.1016/j.molcel.2016.08.021
通讯作者	Zhang, hong(hongzhang@sun5.ibp.ac.cn)
英文摘要	Mutations in the human autophagy gene epg5 cause the multisystem disorder vici syndrome. here we demonstrated that epg5 is a rab7 effector that determines the fusion specificity of autophagosomes with late endosomes/lysosomes. epg5 is recruited to late endosomes/lysosomes by direct interaction with rab7 and the late endosomal/lysosomal r-snare vamp7/8. epg5 also binds to lc3/lgg-1 (mammalian and c. elegans atg8 homolog, respectively) and to assembled stx17-snap29 qabc snare complexes on autophagosomes. epg5 stabilizes and facilitates the assembly of stx17snap29-vamp7/8 trans-snare complexes, and promotes stx17-snap29-vamp7-mediated fusion of reconstituted proteoliposomes. loss of epg5 activity causes abnormal fusion of autophagosomes with various endocytic vesicles, in part due to elevated assembly of stx17-snap25-vamp8 complexes. snap25 knockdown partially suppresses the autophagy defect caused by epg5 depletion. our study reveals that epg5 is a rab7 effector involved in autophagosome maturation, providing insight into the molecular mechanism underlying vici syndrome.
WOS关键词	MEMBRANE-FUSION ; C. ELEGANS ; LYSOSOME FUSION ; HOPS COMPLEX ; SYNTAXIN 17 ; VESICLE ; MATURATION ; SNARE ; MECHANISMS ; MACHINERY
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000382542000008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375651
专题	中国科学院大学
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Peking Univ, Sch Basic Med Sci, Dept Immunol, Beijing 100191, Peoples R China 3.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China 4.Guangdong Med Coll, Affiliated Hosp, Inst Neurol, Zhanjiang 524001, Peoples R China 5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zheng,Miao, Guangyan,Xue, Xue,et al. The vici syndrome protein epg5 is a rab7 effector that determines the fusion specificity of autophagosomes with late endosomes/lysosomes[J]. Molecular cell,2016,63(5):781-795.
APA	Wang, Zheng.,Miao, Guangyan.,Xue, Xue.,Guo, Xiangyang.,Yuan, Chongzhen.,...&Zhang, Hong.(2016).The vici syndrome protein epg5 is a rab7 effector that determines the fusion specificity of autophagosomes with late endosomes/lysosomes.Molecular cell,63(5),781-795.
MLA	Wang, Zheng,et al."The vici syndrome protein epg5 is a rab7 effector that determines the fusion specificity of autophagosomes with late endosomes/lysosomes".Molecular cell 63.5(2016):781-795.

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