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	Expression of v-cath gene from hearnpv in tobacco confers an antifeedant effect against helicoverpa armigera
	Zhang, Yong1,2; Ma, Fang1,2; Wang, Yao1; Yang, Baoyu1; Chen, Shiyun1
刊名	Journal of biotechnology
	2008-11-06
卷号	138期号:1-2页码:52-55
关键词	Antifeedant V-cath gene Baculovirus Hearnpv Helicoverpa armigera Transgenic plant
ISSN号	0168-1656
DOI	10.1016/j.jbiotec.2008.07.1990
通讯作者	Chen, shiyun(sychen@wh.iov.cn)
英文摘要	Biotechnology solutions for insect control on crops largely depend on the expression of bacillus thuringiensis insecticidal proteins to kill pests. v-cath, a cathepsin l-like cysteine protease from baculoviruses, has been shown to play an essential role in host insect liquefaction. in this study, the v-cath gene from helicoverpa armigera single nucleopolyhedrovirus (hearnpv) was cloned into the pbi121 binary vector under the control of camv35s promoter, and was expressed in tobacco via agrobacterium-mediated transformation. pcr and rt-pcr analyses of t-1 kanamycin-resistant tobacco progeny plants confirmed the integration and transcription of the v-cath gene. using a leaf-disk bioassay, antifeedant activity toward h. armigera was tested. our result showed that, when feeding the first-instar larvae of h. armigera with leaves of transgenic plants, the v-cath transgene expression has a profound antifeedant effect. most importantly, the growth and development of the insect were inhibited when transferred from leaf-feeding to artificial diet. our result demonstrated that v-cath gene from baculovirus induced antifeedant effect against h. armigera. resulted in larval stunting and retarded insect development, and has the potential to be used as an alternative way to generate transgenic plants for insect pest control. (c) 2008 elsevier b.v. all rights reserved.
WOS关键词	INSECT PEST-CONTROL ; RESISTANCE ; NUCLEOPOLYHEDROVIRUS ; BACULOVIRUS ; BOLLWORM ; PLANTS ; COTTON ; IDENTIFICATION ; LEPIDOPTERA ; GENOME
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000261077000007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375472
专题	武汉病毒研究所
通讯作者	Chen, Shiyun
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei Province, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yong,Ma, Fang,Wang, Yao,et al. Expression of v-cath gene from hearnpv in tobacco confers an antifeedant effect against helicoverpa armigera[J]. Journal of biotechnology,2008,138(1-2):52-55.
APA	Zhang, Yong,Ma, Fang,Wang, Yao,Yang, Baoyu,&Chen, Shiyun.(2008).Expression of v-cath gene from hearnpv in tobacco confers an antifeedant effect against helicoverpa armigera.Journal of biotechnology,138(1-2),52-55.
MLA	Zhang, Yong,et al."Expression of v-cath gene from hearnpv in tobacco confers an antifeedant effect against helicoverpa armigera".Journal of biotechnology 138.1-2(2008):52-55.

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