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	Effects of quorum sensing autoinducer degradation gene on virulence and biofilm formation of pseudomonas aeruginosa
	Wang Yao; Dai Yue; Zhang Yong; Hu YangBo; Yang BaoYu; Chen ShiYun
刊名	Science in china series c-life sciences
	2007-06-01
卷号	50期号:3页码:385-391
关键词	Pseudomonas aeruginosa Aiia Virulence factors Biofilm Quorum sensing
ISSN号	1006-9305
DOI	10.1007/s11427-007-0044-y
通讯作者	Chen shiyun(sychen@wh.iov.cn)
英文摘要	The aiia gene from bacillus thuringiensis was cloned into the pseudomonas/e. coli shuttle vector and transformed into pseudomonas aeruginosa strain pao1. western blotting showed that the aha protein was expressed in pao1. after induction by iptg for 6 h and 18 h, expression of the aiia gene in pao1 completely degraded the quorum sensing autoinducers n-acylhomoserine lactones (ahls): n-oxododecanoyl-l-homoserine lactone (oddhl) and n-butyryl-l-homoserine lactone (bhl). the reduced amount of ahls in pao1 was also correlated with decreased expression and production of several virulence factors such as elastase and pyocyanin. aha expression also influenced bacterial swarming motility. most importantly, our studies indicated that aiia played significant roles in p. aeruginosa biofilm formation and dispersion, as observed by the differences of the biofilm formation on liquid and solid surfaces, and biofilm structures under a scanning electron microscope.
WOS关键词	BACILLUS-THURINGIENSIS ; ERWINIA-CAROTOVORA ; IN-VIVO ; ENZYME ; SIGNAL ; INFECTIONS ; INHIBITION ; APOPTOSIS ; BACTERIA ; MOTILITY
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000247829500014
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375421
专题	武汉病毒研究所
通讯作者	Chen ShiYun
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang Yao,Dai Yue,Zhang Yong,et al. Effects of quorum sensing autoinducer degradation gene on virulence and biofilm formation of pseudomonas aeruginosa[J]. Science in china series c-life sciences,2007,50(3):385-391.
APA	Wang Yao,Dai Yue,Zhang Yong,Hu YangBo,Yang BaoYu,&Chen ShiYun.(2007).Effects of quorum sensing autoinducer degradation gene on virulence and biofilm formation of pseudomonas aeruginosa.Science in china series c-life sciences,50(3),385-391.
MLA	Wang Yao,et al."Effects of quorum sensing autoinducer degradation gene on virulence and biofilm formation of pseudomonas aeruginosa".Science in china series c-life sciences 50.3(2007):385-391.

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