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	Rapid high throughput assay for fluorimetric detection of doxorubicin - application of nucleic acid-dye bioprobe
	Liu, Yang; Danielsson, Bengt
刊名	Analytica chimica acta
	2007-03-21
卷号	587期号:1页码:47-51
关键词	Doxorubicin Fluorescence Deoxyribonucleic acid High throughput
ISSN号	0003-2670
DOI	10.1016/j.aca.2007.01.013
通讯作者	Danielsson, bengt(bengt.danielsson@tbiokem.lth.se)
英文摘要	A double stranded dna based fluorescence bioprobe for anticancer agent (doxorubicin) detection is described. this method provides a new way for sensitive dna/drug interaction study by a homogeneous assay. the probe employs the long-wavelength intercalating fluorophore toto-3((r)) (tt3). the anticancer agent, doxorubicin, which interacts with the dna-tt3 complex, was indirectly measured by the decrease in the fluorescence intensity. various oligonucleotides with different sequences were examined. doxorubicin has preference for the oligonucleotide 5'agcacg3'. enhanced fluorescence observed for the tt3 intercalation with this oligonucleotide makes the dna-dye complex a suitable bioprobe for doxorubicin detection by competitive assay. a home-built ccd camera setup was applied along with 384 well plate assay format for high throughput fluorescence imaging. the detection limit can be as low as 25 ng ml(-1) with an upper limit of 100 mu g ml(-1). the recovery test with spiked serum sample shows that this method can be a potential routine method for therapeutic drug monitoring (tdm). (c) 2007 elsevier b.v. all rights reserved.
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; DNA PROBES ; ANTHRACYCLINE DERIVATIVES ; NMR-SPECTROSCOPY ; ADRIAMYCIN ; BINDING ; DAUNOMYCIN ; ANTIBIOTICS ; SPECIFICITY ; METABOLITES
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000245158700007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375368
专题	武汉病毒研究所
通讯作者	Danielsson, Bengt
作者单位	1.Lund Univ, SE-22100 Lund, Sweden 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yang,Danielsson, Bengt. Rapid high throughput assay for fluorimetric detection of doxorubicin - application of nucleic acid-dye bioprobe[J]. Analytica chimica acta,2007,587(1):47-51.
APA	Liu, Yang,&Danielsson, Bengt.(2007).Rapid high throughput assay for fluorimetric detection of doxorubicin - application of nucleic acid-dye bioprobe.Analytica chimica acta,587(1),47-51.
MLA	Liu, Yang,et al."Rapid high throughput assay for fluorimetric detection of doxorubicin - application of nucleic acid-dye bioprobe".Analytica chimica acta 587.1(2007):47-51.

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