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	Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip
	Li, Yong-Jin; Bi, Li-Jun; Zhang, Xian-En; Zhou, Ya-Feng; Zhang, Ji-Bin; Chen, Yuan-Yuan; Li, Wei; Zhang, Zhi-Ping
刊名	Analytical and bioanalytical chemistry
	2006-11-01
卷号	386期号:5页码:1321-1326
关键词	Surface plasmon resonance Streptavidin affinity tag Nano-tag Site-directed immobilization Regeneration
ISSN号	1618-2642
DOI	10.1007/s00216-006-0794-6
通讯作者	Zhang, xian-en(zhangxe@most.cn)
英文摘要	Dissociation of biotin from streptavidin is very difficult due to their high binding affinity. the re-use of streptavidin-modified surfaces is therefore almost impossible, making devices containing them (e.g. surface plasmon resonance (spr) sensor chips) expensive. this paper describes a new protocol for reversible and site-directed immobilization of proteins with streptavidin affinity tags on the streptavidin-coated spr biosensor chip (sa chip). two streptavidin affinity tags, nano-tag and streptavidin-binding peptide (sbp tag), were applied. they both can specifically interact with streptavidin but have weaker binding force compared to the biotin-streptavidin system, thus allowing association and dissociation under controlled conditions. the sa chip surface could be regenerated repeatedly without loss of activity by injection of 50 mm naoh solution. the fusion construct of a sbp tag and a single-chain antibody to mature bovine prion protein (scfv-z186-sbp) interacts with the sa chip, resulting in a single-chain-antibody-modified surface. the chip showed kinetic response to the prion antigen with equilibrium dissociation constant k (d)approximate to 4.01x10(-7)supercript stop. all results indicated that the capture activity of the sa chip has no irreversible loss after repeated immobilization and regeneration cycles. the method should be of great benefit to various biosensors, biochips and immunoassay applications based on the streptavidin capture surface.
WOS关键词	RECOMBINANT PROTEINS ; BINDING PEPTIDE ; PURIFICATION ; SENSORS ; SYSTEM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000241604700017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375328
专题	武汉病毒研究所
通讯作者	Zhang, Xian-En
作者单位	1.Chinese Acad Sci, Inst Biophys, State Key Lab Macromol, Joint Res Grp Analyt Biotechnol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Yong-Jin,Bi, Li-Jun,Zhang, Xian-En,et al. Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip[J]. Analytical and bioanalytical chemistry,2006,386(5):1321-1326.
APA	Li, Yong-Jin.,Bi, Li-Jun.,Zhang, Xian-En.,Zhou, Ya-Feng.,Zhang, Ji-Bin.,...&Zhang, Zhi-Ping.(2006).Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip.Analytical and bioanalytical chemistry,386(5),1321-1326.
MLA	Li, Yong-Jin,et al."Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip".Analytical and bioanalytical chemistry 386.5(2006):1321-1326.

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