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	Response of crayfish, procambarus clarkii, haemocytes infected by white spot syndrome virus
	Shi, Z; Wang, H; Zhang, J; Xie, Y; Li, L; Chen, X; Edgerton, BF; Bonami, JR
刊名	Journal of fish diseases
	2005-03-01
卷号	28期号:3页码:151-156
关键词	Crayfish Electron microscopy Haemocyte response In situ hybridization Procambarus clarkii White spot syndrome virus
ISSN号	0140-7775
通讯作者	Shi, z(zlshi@pentium.whiov.ac.cn)
英文摘要	White spot syndrome virus ( wssv) is a serious pathogen of aquatic crustaceans. little is known about its transmission in vivo and the immune reaction of its hosts. in this study, the circulating haemocytes of crayfish, procambarus clarkii, infected by wssv, and primary haemocyte cultures inoculated with wssv, were collected and observed by transmission electron microscopy and light microscopy following in situ hybridization. in ultrathin sections of infected haemocytes, the enveloped virions were seen to be phagocytosed in the cytoplasm and no viral particles were observed in the nuclei. in situ hybridization with wssv-specific probes also demonstrated that there were no specific positive signals present in the haemocytes. conversely, strong specific positive signals showed that wssv replicated in the nuclei of gill cells. as a control, the lymphoid organ of shrimp, penaeus monodon, infected by wssv was examined by in situ hybridization which showed that wssv did not replicate within the tubules of the lymphoid organ. in contrast to previous studies, it is concluded that neither shrimp nor crayfish haemocytes support wssv replication.white spot syndrome virus (wssv) is a serious pathogen of aquatic crustaceans. little is known about its transmission in vivo and the immune reaction of its hosts. in this study, the circulating haemocytes of crayfish, procambarus clarkii, infected by wssv, and primary haemocyte cultures inoculated with wssv, were collected and observed by transmission electron microscopy and light microscopy following in situ hybridization. in ultra-thin sections of infected haemocytes, the enveloped virions were seen to be phagocytosed in the cytoplasm and no viral particles were observed in the nuclei. in situ hybridization with wssv-specific probes also demonstrated that there were no specific positive signals present in the haemocytes. conversely, strong specific positive signals showed that wssv replicated in the nuclei of gill cells. as a control, the lymphoid organ of shrimp, penaeus monodon, infected by wssv was examined by in situ hybridization which showed that wssv did not replicate within the tubules of the lymphoid organ. in contrast to previous studies, it is concluded that neither shrimp nor crayfish haemocytes support wssv replication.
WOS关键词	SYNDROME BACULOVIRUS WSBV ; SHRIMP PENAEUS-MONODON ; GENOME SEQUENCE ; ULTRASTRUCTURE ; SYSTEM ; TISSUE ; CELLS ; GENE ; WSSV
WOS研究方向	Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	BLACKWELL PUBLISHING LTD
WOS记录号	WOS:000227479700004
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375222
专题	武汉病毒研究所
通讯作者	Shi, Z
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Mol Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 3.Univ Queensland, Ctr Marine Sci, St Lucia, Qld, Australia 4.Univ Montpellier 2, EcoLag, Montpellier 5, France
推荐引用方式 GB/T 7714	Shi, Z,Wang, H,Zhang, J,et al. Response of crayfish, procambarus clarkii, haemocytes infected by white spot syndrome virus[J]. Journal of fish diseases,2005,28(3):151-156.
APA	Shi, Z.,Wang, H.,Zhang, J.,Xie, Y.,Li, L.,...&Bonami, JR.(2005).Response of crayfish, procambarus clarkii, haemocytes infected by white spot syndrome virus.Journal of fish diseases,28(3),151-156.
MLA	Shi, Z,et al."Response of crayfish, procambarus clarkii, haemocytes infected by white spot syndrome virus".Journal of fish diseases 28.3(2005):151-156.

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