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	Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy
	Qu, XX; Hao, P; Song, XJ; Jiang, SM; Liu, YX; Wang, PG; Rao, X; Song, HD; Wang, SY; Zuo, Y
刊名	Journal of biological chemistry
	2005-08-19
卷号	280期号:33页码:29588-29595
ISSN号	0021-9258
DOI	10.1074/jbc.m500662200
通讯作者	Zhao, gp()
英文摘要	Severe acute respiratory syndrome coronavirus (sars-cov) is a recently identified human coronavirus. the extremely high homology of the viral genomic sequences between the viruses isolated from human (husars-cov) and those of palm civet origin (pcsarscov) suggested possible palm civet-to-human transmission. genetic analysis revealed that the spike ( s) protein of pcsars-cov and husars-cov was subjected to the strongest positive selection pressure during transmission, and there were six amino acid residues within the receptor-binding domain of the s protein being potentially important for sars progression and tropism. using the single-round infection assay, we found that a two-amino acid substitution (n479k/t487s) of a husars-cov for those of pcsarscov almost abolished its infection of human cells expressing the sars-cov receptor ace2 but no effect upon the infection of mouse ace2 cells. although single substitution of these two residues had no effects on the infectivity of husars-cov, these recombinant s proteins bound to human ace2 with different levels of reduced affinity, and the two-amino acid-substituted s protein showed extremely low affinity. on the contrary, substitution of these two amino acid residues of pcsars-cov for those of husras-cov made pcsars-cov capable of infecting human ace2-expressing cells. these results suggest that amino acid residues at position 479 and 487 of the s protein are important determinants for sars-cov tropism and animal-to-human transmission.
WOS关键词	ANGIOTENSIN-CONVERTING ENZYME-2 ; SARS-CORONAVIRUS ; AMINOPEPTIDASE-N ; FUNCTIONAL-CHARACTERIZATION ; NEUTRALIZING ANTIBODIES ; EFFICIENT REPLICATION ; VIRUS TYPE-1 ; RECEPTOR ; GLYCOPROTEIN ; EXPRESSION
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000231176200026
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375200
专题	武汉病毒研究所
通讯作者	Zhao, GP
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Ctr Hlth Sci, Bioinformat Ctr,Inst Plant Physiol & Ecol, Shanghai 200031, Peoples R China 2.Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China 3.Shanghai Med Univ 2, Ruijin Hosp, Pole Sinfrancais Rech Sci Vivant & Genom, State Key Lab Med Genom, Shanghai 200025, Peoples R China 4.Chinese Natl Human Genome Ctr, Shanghai 201203, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 6.Fudan Univ, Sch Life Sci, Dept Microbiol, State Key lab Genet Engn, Shanghai 200433, Peoples R China
推荐引用方式 GB/T 7714	Qu, XX,Hao, P,Song, XJ,et al. Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy[J]. Journal of biological chemistry,2005,280(33):29588-29595.
APA	Qu, XX.,Hao, P.,Song, XJ.,Jiang, SM.,Liu, YX.,...&Deng, HK.(2005).Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy.Journal of biological chemistry,280(33),29588-29595.
MLA	Qu, XX,et al."Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy".Journal of biological chemistry 280.33(2005):29588-29595.

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