| Structural characterization of coatomer in its cytosolic state | |
| Wang, Shengliu1,4; Zhai, Yujia1; Pang, Xiaoyun1; Niu, Tongxin1,4; Ding, Yue-He2; Dong, Meng-Qiu2; Hsu, Victor W.5; Sun, Zhe1; Sun, Fei1,3,4 | |
| 刊名 | Protein & cell
 |
| 2016-08-01 | |
| 卷号 | 7期号:8页码:586-600 |
| 关键词 | Coatomer Copi Human Single-particle electron microscopy Membrane trafficking |
| ISSN号 | 1674-800X |
| DOI | 10.1007/s13238-016-0296-z |
| 通讯作者 | Sun, zhe(zsun@moon.ibp.ac.cn) ; Sun, fei(feisun@ibp.ac.cn) |
| 英文摘要 | Studies on coat protein i (copi) have contributed to a basic understanding of how coat proteins generate vesicles to initiate intracellular transport. the core component of the copi complex is coatomer, which is a multimeric complex that needs to be recruited from the cytosol to membrane in order to function in membrane bending and cargo sorting. previous structural studies on the clathrin adaptors have found that membrane recruitment induces a large conformational change in promoting their role in cargo sorting. here, pursuing negative-stain electron microscopy coupled with single-particle analyses, and also performing cxms (chemical cross-linking coupled with mass spectrometry) for validation, we have reconstructed the structure of coatomer in its soluble form. when compared to the previously elucidated structure of coatomer in its membrane-bound form we do not observe a large conformational change. thus, the result uncovers a key difference between how copi versus clathrin coats are regulated by membrane recruitment. |
| WOS关键词 | PARTICLE ELECTRON CRYOMICROSCOPY ; PUTATIVE CARGO RECEPTORS ; CLATHRIN ADAPTER COMPLEX ; COATED VESICLE FORMATION ; ADP-RIBOSYLATION FACTOR ; GTP-BINDING PROTEIN ; MEMBRANE CURVATURE ; COPI COAT ; CONFORMATIONAL-CHANGE ; EXCHANGE FACTOR |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| 出版者 | HIGHER EDUCATION PRESS |
| WOS记录号 | WOS:000381583800005 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375195 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, Zhe; Sun, Fei |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China 2.Natl Inst Biol Sci, Beijing 102206, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Ctr Biol Imaging, Beijing 100101, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Boston, MA 02115 USA |
| 推荐引用方式 GB/T 7714 | Wang, Shengliu,Zhai, Yujia,Pang, Xiaoyun,et al. Structural characterization of coatomer in its cytosolic state[J]. Protein & cell,2016,7(8):586-600. |
| APA | Wang, Shengliu.,Zhai, Yujia.,Pang, Xiaoyun.,Niu, Tongxin.,Ding, Yue-He.,...&Sun, Fei.(2016).Structural characterization of coatomer in its cytosolic state.Protein & cell,7(8),586-600. |
| MLA | Wang, Shengliu,et al."Structural characterization of coatomer in its cytosolic state".Protein & cell 7.8(2016):586-600. |
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