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Purification and characterization of a new reovirus from the chinese mitten crab, eriocheir sinensis
Zhang, S; Shi, Z; Zhang, J; Bonami, JR
刊名Journal of fish diseases
2004-12-01
卷号27期号:12页码:687-692
关键词Characterization Chinese mitten crab Eriocheir sinensis Purification Reovirus Ultrastructure
ISSN号0140-7775
通讯作者Bonami, jr()
英文摘要A new reovirus was recently isolated from a freshwater crab, the chinese mitten crab, eriocheir sinensis, in china. the complete viral particles are 55 nm in diameter, icosahedral, non-enveloped and have a mean buoyant density of 1.39 g cm(-3) in cscl gradient. the viral genome is composed of 12 pieces of dsrna with an electrophoretic pattern of 3/4/2/3. this virus infects connective tissue of the gills, gut and hepatopancreas. partial cdna cloning and sequence analysis showed that the rna-dependent rna polymerase is located in the first rna segment. from its biochemical, ultrastructural and physicochemical properties, this virus is quite different from the genus aquareovirus (reoviridae). it may represent a new genus of reoviridae, different from the other crab reoviruses, p and w-2.
WOS关键词TREMOR DISEASE
WOS研究方向Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
出版者BLACKWELL PUBLISHING LTD
WOS记录号WOS:000225487200002
内容类型期刊论文
URI标识http://www.corc.org.cn/handle/1471x/2375190
专题武汉病毒研究所
通讯作者Bonami, JR
作者单位1.Univ Montpellier 2, CNRS, UMR 5119, Ecolag, F-34095 Montpellier 5, France
2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan, Peoples R China
推荐引用方式
GB/T 7714
Zhang, S,Shi, Z,Zhang, J,et al. Purification and characterization of a new reovirus from the chinese mitten crab, eriocheir sinensis[J]. Journal of fish diseases,2004,27(12):687-692.
APA Zhang, S,Shi, Z,Zhang, J,&Bonami, JR.(2004).Purification and characterization of a new reovirus from the chinese mitten crab, eriocheir sinensis.Journal of fish diseases,27(12),687-692.
MLA Zhang, S,et al."Purification and characterization of a new reovirus from the chinese mitten crab, eriocheir sinensis".Journal of fish diseases 27.12(2004):687-692.
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