Purification and characterization of a new reovirus from the chinese mitten crab, eriocheir sinensis | |
Zhang, S; Shi, Z; Zhang, J; Bonami, JR | |
刊名 | Journal of fish diseases |
2004-12-01 | |
卷号 | 27期号:12页码:687-692 |
关键词 | Characterization Chinese mitten crab Eriocheir sinensis Purification Reovirus Ultrastructure |
ISSN号 | 0140-7775 |
通讯作者 | Bonami, jr() |
英文摘要 | A new reovirus was recently isolated from a freshwater crab, the chinese mitten crab, eriocheir sinensis, in china. the complete viral particles are 55 nm in diameter, icosahedral, non-enveloped and have a mean buoyant density of 1.39 g cm(-3) in cscl gradient. the viral genome is composed of 12 pieces of dsrna with an electrophoretic pattern of 3/4/2/3. this virus infects connective tissue of the gills, gut and hepatopancreas. partial cdna cloning and sequence analysis showed that the rna-dependent rna polymerase is located in the first rna segment. from its biochemical, ultrastructural and physicochemical properties, this virus is quite different from the genus aquareovirus (reoviridae). it may represent a new genus of reoviridae, different from the other crab reoviruses, p and w-2. |
WOS关键词 | TREMOR DISEASE |
WOS研究方向 | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS类目 | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
语种 | 英语 |
出版者 | BLACKWELL PUBLISHING LTD |
WOS记录号 | WOS:000225487200002 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2375190 |
专题 | 武汉病毒研究所 |
通讯作者 | Bonami, JR |
作者单位 | 1.Univ Montpellier 2, CNRS, UMR 5119, Ecolag, F-34095 Montpellier 5, France 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, S,Shi, Z,Zhang, J,et al. Purification and characterization of a new reovirus from the chinese mitten crab, eriocheir sinensis[J]. Journal of fish diseases,2004,27(12):687-692. |
APA | Zhang, S,Shi, Z,Zhang, J,&Bonami, JR.(2004).Purification and characterization of a new reovirus from the chinese mitten crab, eriocheir sinensis.Journal of fish diseases,27(12),687-692. |
MLA | Zhang, S,et al."Purification and characterization of a new reovirus from the chinese mitten crab, eriocheir sinensis".Journal of fish diseases 27.12(2004):687-692. |
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