| Biometallization-based electrochemical magnetoimmunosensing strategy for avian influenza a (h7n9) virus particle detection | |
| Zhou, Chuan-Hua1,2; Wu, Zhen1; Chen, Jian-Jun3; Xiong, Chaochao3; Chen, Ze4; Pang, Dai-Wen1; Zhang, Zhi-Ling1 | |
| 刊名 | Chemistry-an asian journal
 |
| 2015-06-01 | |
| 卷号 | 10期号:6页码:1387-1393 |
| 关键词 | Avian influenza a (h7n9) virus Bi-electrode signal transduction Biometallization Electrochemical immunosensors Immunoassays Magnetic beads |
| ISSN号 | 1861-4728 |
| DOI | 10.1002/asia.201500105 |
| 通讯作者 | Chen, jian-jun(chenjj126@126.com) |
| 英文摘要 | A highly sensitive electrochemical immunosensor for avian influenza a (h7n9) virus (h7n9 aiv) detection was proposed by using electrochemical magnetoimmunoassay coupled with biometallization and anodic stripping voltammetry. this strategy could accumulate the enzyme-generated product on the surface of the magneto electrode by means of silver deposition, which amplified the detection signal about 80 times. the use of magnetic beads (mbs) and the magneto electrode could also amplify the detection signal. furthermore, a bi-electrode signal transduction system was introduced into this immunosensor, which is also beneficial to the immunoassay. a concentration as low as 0.011ngml(-1) of h7n9 aiv could be detected in about 1.5h with good specificity. this study not only provides a simple and sensitive approach for virus detection but also offers an effective signal enhancement strategy for the development of highly sensitive mb-based electrochemical immunoassays. |
| WOS关键词 | MAGNETIC NANOPARTICLES ; ELECTRICAL DETECTION ; METAL-DEPOSITION ; RAPID DETECTION ; AMPLIFICATION ; IMMUNOSENSOR ; DNA ; IMMUNOASSAY ; ANTIGEN ; SERUM |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000355339100018 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375181 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Jian-Jun |
| 作者单位 | 1.Wuhan Univ, Coll Chem & Mol Sci, State Key Lab Virol, Key Lab Analyt Chem Biol & Med,Minist Educ, Wuhan 430072, Peoples R China 2.Yunnan Univ, Sch Chem Sci & Technol, Key Lab Med Chem Nat Resource, Minist Educ, Kunming 650091, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China 4.Shanghai Inst Biol Prod, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Chuan-Hua,Wu, Zhen,Chen, Jian-Jun,et al. Biometallization-based electrochemical magnetoimmunosensing strategy for avian influenza a (h7n9) virus particle detection[J]. Chemistry-an asian journal,2015,10(6):1387-1393. |
| APA | Zhou, Chuan-Hua.,Wu, Zhen.,Chen, Jian-Jun.,Xiong, Chaochao.,Chen, Ze.,...&Zhang, Zhi-Ling.(2015).Biometallization-based electrochemical magnetoimmunosensing strategy for avian influenza a (h7n9) virus particle detection.Chemistry-an asian journal,10(6),1387-1393. |
| MLA | Zhou, Chuan-Hua,et al."Biometallization-based electrochemical magnetoimmunosensing strategy for avian influenza a (h7n9) virus particle detection".Chemistry-an asian journal 10.6(2015):1387-1393. |
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