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	Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection
	Chai, Fan1,2; Li, Hao-Yu1,3; Wang, Wei1,2; Zhu, Xiu-Juan1,3; Li, Yang1,3; Wang, Shaobo1,2; Guo, Lin1,3; Zhang, Lei-Ke1,2; Xiao, Gengfu1,2
刊名	Biochimica et biophysica acta-proteins and proteomics
	2015-08-01
卷号	1854期号:8页码:967-978
关键词	Human cytomegalovirus Quantitative proteomics Sirna
ISSN号	1570-9639
DOI	10.1016/j.bbapap.2015.04.016
通讯作者	Zhang, lei-ke(zhangleike@wh.iov.cn)
英文摘要	Viral replication requires host cell macromolecules and energy, although host cells can alter their protein expression to restrict viral replication. to study the host cell response to human cytomegalovirus (hcmv) infection, a stable isotope labeling by amino adds in cell culture (silac)-based subcellular quantitative proteomic study of hcmv-infected human embryo lung fibroblast (hel) cells was performed, and a total of 247 host proteins were identified as differentially regulated by hcmv. western blotting and immunofluorescence confocal microscopy were performed to validate the data sets. gene ontology analysis indicated that cellular processes involving the metabolism, localization and immune system were regulated as a result of hcmv infection. functional analysis of selected regulated proteins revealed that knockdown of hnrpd, phb2 and ub2v2 can increase hcmv replication, while knockdown of a4 and ksrp resulted in decreased hcmv replication. our study may improve our understanding of the dynamic interactions between hcmv and its host and provide multiple potential targets for anti-hcmv agent research. (c) 2015 elsevier b.v. all rights reserved.
WOS关键词	JAPANESE ENCEPHALITIS-VIRUS ; GENE-EXPRESSION ; INTERFERON RESPONSE ; CELLS ; INDUCTION ; IDENTIFICATION ; NUCLEAR ; DISEASE ; RNA ; DNA
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000356642600013
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375171
专题	武汉病毒研究所
通讯作者	Zhang, Lei-Ke
作者单位	1.Chinese Acad Sci, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 3.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Chai, Fan,Li, Hao-Yu,Wang, Wei,et al. Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection[J]. Biochimica et biophysica acta-proteins and proteomics,2015,1854(8):967-978.
APA	Chai, Fan.,Li, Hao-Yu.,Wang, Wei.,Zhu, Xiu-Juan.,Li, Yang.,...&Xiao, Gengfu.(2015).Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.Biochimica et biophysica acta-proteins and proteomics,1854(8),967-978.
MLA	Chai, Fan,et al."Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection".Biochimica et biophysica acta-proteins and proteomics 1854.8(2015):967-978.

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