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	Peptide biosynthesis with stable isotope labeling from a cell-free expression system for targeted proteomics with absolute quantification
	Xian, Feng1,2,3; Zi, Jin2; Wang, Quanhui1,2; Lou, Xiaomin1; Sun, Haidan1; Lin, Liang2; Hou, Guixue1,2; Rao, Weiqiao2; Yin, Changcheng5; Wu, Lin1
刊名	Molecular & cellular proteomics
	2016-08-01
卷号	15期号:8页码:2819-2828
ISSN号	1535-9476
DOI	10.1074/mcp.o115.056507
通讯作者	Li, shuwei(sli@umd.edu) ; Liu, siqi(siqiliu@genomics.cn)
英文摘要	Because of its specificity and sensitivity, targeted proteomics using mass spectrometry for multiple reaction monitoring is a powerful tool to detect and quantify pre-selected peptides from a complex background and facilitates the absolute quantification of peptides using isotope-labeled forms as internal standards. how to generate isotope-labeled peptides remains an urgent challenge for accurately quantitative targeted proteomics on a large scale. herein, we propose that isotope-labeled peptides fused with a quantitative tag could be synthesized through an expression system in vitro, and the homemade peptides could be enriched by magnetic beads with tag-affinity and globally quantified based on the corresponding multiple reaction monitoring signals provided by the fused tag. an escherichia coli cell-free protein expression system, protein synthesis using recombinant elements, was adopted for the synthesis of isotope-labeled peptides fused with strep-tag. through a series of optimizations, we enabled efficient expression of the labeled peptides such that, after strep-tactin affinity enrichment, the peptide yield was acceptable in scale for quantification, and the peptides could be completely digested by trypsin to release the strep-tag for quantification. moreover, these recombinant peptides could be employed in the same way as synthetic peptides for multiple reaction monitoring applications and are likely more economical and useful in a laboratory for the scale of targeted proteomics. as an application, we synthesized four isotope-labeled glutathione s-transferase (gst) peptides and added them to mouse sera pre-treated with gst affinity resin as internal standards. a quantitative assay of the synthesized gst peptides confirmed the absolute gst quantification in mouse sera to be measurable and reproducible.
WOS关键词	CONCATENATED SIGNATURE PEPTIDES ; GLUTATHIONE S-TRANSFERASES ; ESCHERICHIA-COLI ; PROTEINS ; EXTRACT ; GENES
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000380809100021
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375089
专题	中国科学院大学
通讯作者	Li, Shuwei; Liu, Siqi
作者单位	1.Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China 2.BGI Shenzhen, Shenzhen 518083, Peoples R China 3.Univ Chinese Acad Sci, Sino Danish Ctr Educ & Res, Beijing 100049, Peoples R China 4.Univ Maryland, Inst Biosci & Biotechnol Res, Rockville, MD 20850 USA 5.Beijing Prot Innovat, Beijing 101318, Peoples R China
推荐引用方式 GB/T 7714	Xian, Feng,Zi, Jin,Wang, Quanhui,et al. Peptide biosynthesis with stable isotope labeling from a cell-free expression system for targeted proteomics with absolute quantification[J]. Molecular & cellular proteomics,2016,15(8):2819-2828.
APA	Xian, Feng.,Zi, Jin.,Wang, Quanhui.,Lou, Xiaomin.,Sun, Haidan.,...&Liu, Siqi.(2016).Peptide biosynthesis with stable isotope labeling from a cell-free expression system for targeted proteomics with absolute quantification.Molecular & cellular proteomics,15(8),2819-2828.
MLA	Xian, Feng,et al."Peptide biosynthesis with stable isotope labeling from a cell-free expression system for targeted proteomics with absolute quantification".Molecular & cellular proteomics 15.8(2016):2819-2828.

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