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	Initial detection of the quorum sensing autoinducer activity in the rumen of goats in vivo and in vitro
	Ran Tao1,2; Zhou Chuan-she1,3; Xu Li-wei1; Geng Mei-mei1; Tan Zhi-liang1,3; Tang Shao-xun1,3; Wang Min1,3; Han Xue-feng1,4; Kang Jin-he1
刊名	Journal of integrative agriculture
	2016
卷号	15期号:10页码:2343-2352
关键词	Quorum sensing Ahls Ai-2 Luxs Rumen bacteria Goat
ISSN号	2095-3119
DOI	10.1016/s2095-3119(16)61417-x
通讯作者	Tan zhi-liang(zltan@isa.ac.cn)
英文摘要	Quorum sensing (qs) is a type of microbe-microbe communication system that is widespread among the microbial world, particularly among microorganisms that are symbiotic with plants and animals. thereby, the cell-cell signalling is likely to occur in an anaerobic rumen environment, which is a complex microbial ecosystem. in this study, using six ruminally fistulated liuyang black goats as experimental animals, we aimed to detect the activity of quorum sensing autoinducers (ai) both in vivo and in vitro and to clone the luxs gene that encoded autoinducer-2 (ai-2) synthase of microbial samples that were collected from the rumen of goats. neutral detergent fiber (ndf) and soluble starch were the two types of substrates that were used for in vitro fermentation. the fermented fluid samples were collected at 0, 2, 4, 6, 8, 12, 24, 36, and 48 h of incubation. the acyl-homoserine lactones (ahls) activity was determined using gas chromatography-mass spectrometer (gc-ms) analysis. however, none of the rumen fluid extracts that were collected from the goat rumen showed the same or similar fragmentation pattern to ahls standards. meanwhile, the ai-2 activity, assayed using a vibrio harveyi bb170 bioassay, was negative in all samples that were collected from the goat rumen and from in vitro fermentation fluids. our results indicated that the activities of ahls and ai-2 were not detected in the ruminal contents from six goats and in ruminal fluids obtained from in vitro fermentation at different sampling time-points. however, the homologues of luxs in prevotella ruminicola were cloned from in vivo and in vitro ruminal fluids. we concluded that ahls and ai-2 could not be detected in in vivo and in vitro ruminal fluids of goats using the current detection techniques under current dietary conditions. however, the microbes that inhabited the goat rumen had the potential ability to secrete ai-2 signaling molecules and to communicate with each other via ai-2-mediated qs because of the presence of luxs.
WOS关键词	VIBRIO-HARVEYI ; HOMOSERINE LACTONES ; MASS-SPECTROMETRY ; SIGNAL MOLECULES ; BACTERIA ; BIOASSAY ; QUANTIFICATION ; IDENTIFICATION ; COMMUNICATION ; LUMINESCENCE
WOS研究方向	Agriculture
WOS类目	Agriculture, Multidisciplinary
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000385470500017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374902
专题	中国科学院大学
通讯作者	Tan Zhi-liang
作者单位	1.Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Hunan Res Ctr Livestock & Poultry Sci,Inst Subtro, South Cent Expt Stn Anim Nutr & Feed Sci,Minist A, Changsha 410125, Hunan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.CICAPS, Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China 4.Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Ran Tao,Zhou Chuan-she,Xu Li-wei,et al. Initial detection of the quorum sensing autoinducer activity in the rumen of goats in vivo and in vitro[J]. Journal of integrative agriculture,2016,15(10):2343-2352.
APA	Ran Tao.,Zhou Chuan-she.,Xu Li-wei.,Geng Mei-mei.,Tan Zhi-liang.,...&Kang Jin-he.(2016).Initial detection of the quorum sensing autoinducer activity in the rumen of goats in vivo and in vitro.Journal of integrative agriculture,15(10),2343-2352.
MLA	Ran Tao,et al."Initial detection of the quorum sensing autoinducer activity in the rumen of goats in vivo and in vitro".Journal of integrative agriculture 15.10(2016):2343-2352.

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