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	Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system
	Liu, Sha1,2; Zhang, Hongyan1; Liu, Weimin1; Zhou, Bingjiang1; Ma, Qian1,3; Ge, Jiechao1; Wu, Jiasheng1; Wang, Pengfei1
刊名	Rsc advances
	2016
卷号	6期号:70页码:65930-65935
ISSN号	2046-2069
DOI	10.1039/c6ra10396k
通讯作者	Zhang, hongyan(zhanghongyan@mail.ipc.ac.cn)
英文摘要	In our work, we investigated the interactions between a small molecule, folic acid, and biological cells through the interaction of folic acid and folate receptors using a laser scanning confocal imaging-surface plasmon resonance (lsci-spr) system. the changes of spr peaks and cell concentration had good linear relationships, and fluorescence imaging provided further data. the detection limit was as low as 1.0 x 10(3) cells per ml, and linear coefficients were 0.95206, 0.95454, 0.94287, 0.98711, and 0.99228 for mouse lymphoma (l5178y tk+/-) cells, mouse lymphoma (el4) cells, mouse t lymphocytes (cl.ly 1+2-/9) cells, human lung cancer (a549) cells, and human oral epidermis carcinoma (kb) cells, respectively. the results indicated that the lsci-spr system has potential future application in analyzing small molecule-biological cell affinity and in acquiring quantitative parameters.
WOS关键词	FOLIC-ACID ; CANCER-CELLS ; BREAST-CANCER ; RECEPTOR EXPRESSION ; ANTICANCER DRUGS ; FOLATE RECEPTORS ; BINDING-KINETICS ; DELIVERY ; PROTEIN ; AU
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000379577800076
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374711
专题	中国科学院大学
通讯作者	Zhang, Hongyan
作者单位	1.Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Univ Petr, Beijing Key Lab Opt Detect Technol Oil & Gas, Beijing 102249, Peoples R China
推荐引用方式 GB/T 7714	Liu, Sha,Zhang, Hongyan,Liu, Weimin,et al. Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system[J]. Rsc advances,2016,6(70):65930-65935.
APA	Liu, Sha.,Zhang, Hongyan.,Liu, Weimin.,Zhou, Bingjiang.,Ma, Qian.,...&Wang, Pengfei.(2016).Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system.Rsc advances,6(70),65930-65935.
MLA	Liu, Sha,et al."Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system".Rsc advances 6.70(2016):65930-65935.

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