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	A comprehensive investigation toward the indicative proteins of bladder cancer in urine: from surveying cell secretomes to verifying urine proteins
	Guo, Jiao1,2; Ren, Yan3; Hou, Guixue1,2; Wen, Bo3; Xian, Feng1,2; Chen, Zhen1; Cui, Ping1; Xie, Yingying1,2; Zi, Jin3; Lin, Liang3
刊名	Journal of proteome research
	2016-07-01
卷号	15期号:7页码:2164-2177
关键词	Urine Bladder cancer 2de Itraq Mrm Biomarker
ISSN号	1535-3893
DOI	10.1021/acs.jproteome.6b00106
通讯作者	Wu, lin(wul@big.ac.cn) ; Lou, xiaomin(louxm@big.ac.cn) ; Liu, siqi(siqiliu@genomics.cn)
英文摘要	Urine is an ideal material to study the cancer related protein biomarkers in bladder, whereas exploration to these candidates is confronting technique challenges. herein, we propose a comprehensive strategy of searching the urine proteins related with bladder cancer. the strategy consists of three core combinations, screening the biomarker candidates in the secreted proteins derived from the bladder cancer cell lines and verifying them in patient urines, defining the differential proteins through two-dimensional electrophoresis (2de) and isobaric tags for relative and absolute quantitation (itraq) coupled with lc-ms/ms, and implementing quantitative proteomics of profiling and targeting analysis. with proteomic survey, a total of 700 proteins were found with their abundance of secreted proteins in cancer cell lines different from normal, while 87 proteins were identified in the urine samples. the multiple reaction monitoring (mrm)-based quantification was adapted in verifying the bladder cancer related proteins in individual urine samples, resulting in 10 differential urine proteins linked with the cancer. of these candidates, receiver operating characteristic analysis revealed that the combination of co3 and ldhb was more sensitive as the cancer indicator than other groups. the discovery of the bladder cancer indicators through our strategy has paved an avenue to further biomarker validation.
WOS关键词	COMPLEMENT COMPONENT 3 ; LUNG-CANCER ; TUMOR-MARKERS ; BTA-STAT ; BIOMARKER ; CARCINOMA ; METABOANALYST ; SURVEILLANCE ; EXPRESSION ; DIAGNOSIS
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000379456400008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374708
专题	中国科学院大学
通讯作者	Wu, Lin; Lou, Xiaomin; Liu, Siqi
作者单位	1.Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.BGI Shenzhen, Prote Div, Shenzhen 518083, Guangdong, Peoples R China 4.Shenzhen Second Peoples Hosp, Shenzhen 518028, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Guo, Jiao,Ren, Yan,Hou, Guixue,et al. A comprehensive investigation toward the indicative proteins of bladder cancer in urine: from surveying cell secretomes to verifying urine proteins[J]. Journal of proteome research,2016,15(7):2164-2177.
APA	Guo, Jiao.,Ren, Yan.,Hou, Guixue.,Wen, Bo.,Xian, Feng.,...&Liu, Siqi.(2016).A comprehensive investigation toward the indicative proteins of bladder cancer in urine: from surveying cell secretomes to verifying urine proteins.Journal of proteome research,15(7),2164-2177.
MLA	Guo, Jiao,et al."A comprehensive investigation toward the indicative proteins of bladder cancer in urine: from surveying cell secretomes to verifying urine proteins".Journal of proteome research 15.7(2016):2164-2177.

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