QQ客服 官方微博 反馈留言

	Crispr-based genome editing and expression control systems in clostridium acetobutylicum and clostridium beijerinckii
	Li, Qi1,2; Chen, Jun1; Minton, Nigel P.3; Zhang, Ying3; Wen, Zhiqiang1; Liu, Jinle1,2; Yang, Haifeng1,2; Zeng, Zhe1,2; Ren, Xiaodan1,2; Yang, Junjie1
刊名	Biotechnology journal
	2016-07-01
卷号	11期号:7页码:961-972
关键词	Clostridium Crispr-cas9 Gene expression Genome editing Nickase
ISSN号	1860-6768
DOI	10.1002/biot.201600053
通讯作者	Yang, sheng(syang@sibs.ac.cn)
英文摘要	Solventogenic clostridia are important industrial microorganisms that produce various chemicals and fuels. effective genetic tools would facilitate physiological studies aimed both at improving our understanding of metabolism and optimizing solvent productivity through metabolic engineering. here we have developed an all-in-one, crispr-based genome editing plasmid, pnickclos, that can be used to achieve successive rounds of gene editing in clostridium acetobutylicum atcc 824 and clostridium beijerinckii ncimb 8052 with efficiencies varying from 6.7% to 100% and 18.8% to 100%, respectively. the plasmid specifies the requisite target-specific guide rna, the gene encoding the streptococcus pyogenes cas9 nickase and the genome editing template encompassing the gene-specific homology arms. it can be used to create single target mutants within three days, with a further two days required for the curing of the pnickclos plasmid ready for a second round of mutagenesis. a s. pyogenes dcas9-mediated gene regulation control system, pdcasclos, was also developed and used in a crispri strategy to successfully repress the expression of spo0a in c. acetobutylicum and c. beijerinckii. the combined application of the established high efficiency crispr-cas9 based genome editing and regulation control systems will greatly accelerate future progress in the understanding and manipulation of metabolism in solventogenic clostridia.
WOS关键词	FERMENTATIVE BUTANOL PRODUCTION ; ESCHERICHIA-COLI ; ATCC 824 ; HOMOLOGOUS RECOMBINATION ; ALLELIC EXCHANGE ; GENE-EXPRESSION ; ACETONE FORMATION ; CAS SYSTEMS ; RNA ; ACTIVATION
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目	Biochemical Research Methods ; Biotechnology & Applied Microbiology
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000379786700011
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374631
专题	中国科学院大学
通讯作者	Yang, Sheng
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Univ Nottingham, Sch Life Sci, BBSRC EPSRC Synthet Biol Res Ctr SBRC, Clostridia Res Grp, Nottingham NG7 2RD, England 4.Shanghai Res & Dev Ctr Ind Biotechnol, Shanghai, Peoples R China 5.Jiangsu Natl Synerget Innovat Ctr Adv Mat, Nanjing, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Li, Qi,Chen, Jun,Minton, Nigel P.,et al. Crispr-based genome editing and expression control systems in clostridium acetobutylicum and clostridium beijerinckii[J]. Biotechnology journal,2016,11(7):961-972.
APA	Li, Qi.,Chen, Jun.,Minton, Nigel P..,Zhang, Ying.,Wen, Zhiqiang.,...&Yang, Sheng.(2016).Crispr-based genome editing and expression control systems in clostridium acetobutylicum and clostridium beijerinckii.Biotechnology journal,11(7),961-972.
MLA	Li, Qi,et al."Crispr-based genome editing and expression control systems in clostridium acetobutylicum and clostridium beijerinckii".Biotechnology journal 11.7(2016):961-972.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。