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	Investigations on the cell metabolomics basis of multidrug resistance from tumor cells by ultra-performance liquid chromatography-mass spectrometry
	Zhang, Ruixing1,2; Zhuang, Xiaoyu1,2; Zong, Li1,2; Liu, Shu1; Liu, Zhiqiang1; Song, Fengrui1
刊名	Analytical and bioanalytical chemistry
	2016-08-01
卷号	408期号:21页码:5843-5854
关键词	Ultra-performance liquid chromatography-mass spectrometry Multidrug resistance Cell metabolomics Tumor
ISSN号	1618-2642
DOI	10.1007/s00216-016-9696-4
通讯作者	Song, fengrui(songfr@ciac.ac.cn)
英文摘要	Although anticancer drug resistance has been linked to high expression of p-glycoprotein and the enhanced dna repair ability, the biochemical process and the underlying mechanisms of drug resistance are not clear. in order to clarify the biochemical mechanisms of drug resistance during anticancer drug treatment, we studied the metabolomics of mcf-7/s and mcf-7/adr cell lines, the coc1 and coc1/ddp cell lines, including the metabolic pathways of multidrug-resistant tumor cells and the changes of endogenous substances in cells. the intracellular metabolites were profiled using ultra-performance liquid chromatography-tandem mass spectrometry (uplc-ms/ms). in this study, 24 biomarkers in mcf-7/adr cells and 15 biomarkers in coc1/ddp cells that are involved in some important metabolic pathways were putatively identified. several metabolic pathways are changed in tumor cells showing drug resistance, such as protein synthesis pathways, cysteine synthesis, the glutamine metabolic pathway, and the ammonia cycle; the first of these are involved in the synthesis of some important proteins including membrane proteins, multidrug resistance-associated proteins, and p-glycoprotein (p-gp). proteins related to drug resistance were overexpressed in multidrug-resistant tumor cells. these proteins depended on energy and play important roles in the emergence of drug resistance. the changes in glutathione and cysteine metabolic pathways showed that the cells can activate related metabolic pathways and reduce the cell apoptosis when they encounter oxidative damage. these findings indicate that drug resistance is likely associated with increased p-gp synthesis and reduced apoptosis of tumor cells.
WOS关键词	BREAST-CANCER-CELLS ; DRUG-RESISTANCE ; LEUKEMIA-CELLS ; MCF-7 ; GLUTATHIONE ; METABOLISM ; GROWTH ; DOXORUBICIN ; POLYAMINES ; SPECTROSCOPY
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000381112700017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374423
专题	中国科学院大学
通讯作者	Song, Fengrui
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, Natl Ctr Mass Spectrometry Changchun, Jilin Prov Key Lab Chinese Med Chem & Mass Spectr, 5625 Renmin St, Changchun 130022, Jilin, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ruixing,Zhuang, Xiaoyu,Zong, Li,et al. Investigations on the cell metabolomics basis of multidrug resistance from tumor cells by ultra-performance liquid chromatography-mass spectrometry[J]. Analytical and bioanalytical chemistry,2016,408(21):5843-5854.
APA	Zhang, Ruixing,Zhuang, Xiaoyu,Zong, Li,Liu, Shu,Liu, Zhiqiang,&Song, Fengrui.(2016).Investigations on the cell metabolomics basis of multidrug resistance from tumor cells by ultra-performance liquid chromatography-mass spectrometry.Analytical and bioanalytical chemistry,408(21),5843-5854.
MLA	Zhang, Ruixing,et al."Investigations on the cell metabolomics basis of multidrug resistance from tumor cells by ultra-performance liquid chromatography-mass spectrometry".Analytical and bioanalytical chemistry 408.21(2016):5843-5854.

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