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	Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells
	Xie, Yajun1,2; Wang, Jinlong1,2; Zhang, Yuanya1,2; Liu, Xiaofei1,2; Wang, Xiaorong1,2; Liu, Kehui1; Huang, Xiahe1; Wang, Yingchun1
刊名	Scientific reports
	2016-08-24
卷号	6页码:10
ISSN号	2045-2322
DOI	10.1038/srep31811
通讯作者	Wang, yingchun(ycwang@genetics.ac.cn)
英文摘要	Protein tyrosine phosphorylation is an important mechanism that regulates cytoskeleton reorganization and cell spreading of migratory cells. a number of cytoskeletal proteins are known to be tyrosine phosphorylated (py) in different cellular processes. however, the profile of py proteins during different stages of cell spreading has not been available. using immunoafffinity enrichment of py proteins coupled with label free quantitative proteomics, we quantitatively identified 447 py proteins in the migratory ecv-304 cells at the early spreading (adhesion) and the active spreading stages. we found that py levels of the majority of the quantified proteins were significantly increased in the active spreading stage compared with the early spreading stage, suggesting that active cell spreading is concomitant with extra tyrosine phosphorylation. the major categories of proteins impacted by tyrosine phosphorylation are involved in cytoskeleton and focal adhesion regulation, protein translation and degradation. our findings, for the first time, dissect the cell spreading-specific py signals from the adhesion induced py signals, and provide a valuable resource for the future mechanistic research regarding the regulation of cell spreading.
WOS关键词	CANCER-CELLS ; MYOSIN-II ; ADHESION ; SUPERVILLIN ; KINASE ; QUANTIFICATION ; ADHESOME ; PAXILLIN ; COFILIN ; BINDING
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000381848000001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374375
专题	中国科学院大学
通讯作者	Wang, Yingchun
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, 1 West Beichen Rd, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xie, Yajun,Wang, Jinlong,Zhang, Yuanya,et al. Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells[J]. Scientific reports,2016,6:10.
APA	Xie, Yajun.,Wang, Jinlong.,Zhang, Yuanya.,Liu, Xiaofei.,Wang, Xiaorong.,...&Wang, Yingchun.(2016).Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells.Scientific reports,6,10.
MLA	Xie, Yajun,et al."Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells".Scientific reports 6(2016):10.

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