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	Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into streptomyces ansochromogenes lead to an improved nikkomycin production
	Liao,Guojian1,2; Li,Jine1,2; Li,Lei1; Yang,Haihua1; Tian,Yuqing1; Tan,Huarong1
刊名	Microbial cell factories
	2010-01-23
卷号	9期号:1
ISSN号	1475-2859
DOI	10.1186/1475-2859-9-6
通讯作者	Tan,huarong(tanhr@sun.im.ac.cn)
英文摘要	Abstractbackgroundnikkomycins are a group of peptidyl nucleoside antibiotics produced by streptomyces ansochromogenes. they are competitive inhibitors of chitin synthase and show potent fungicidal, insecticidal, and acaricidal activities. nikkomycin x and z are the main components produced by s. ansochromogenes. generation of a high-producing strain is crucial to scale up nikkomycins production for further clinical trials.resultsto increase the yields of nikkomycins, an additional copy of nikkomycin biosynthetic gene cluster (35 kb) was introduced into nikkomycin producing strain, s. ansochromogenes 7100. the gene cluster was first reassembled into an integrative plasmid by red/et technology combining with classic cloning methods and then the resulting plasmid(pnik)was introduced into s. ansochromogenes by conjugal transfer. introduction of pnik led to enhanced production of nikkomycins (880 mg l-1, 4 -fold nikkomycin x and 210 mg l-1, 1.8-fold nikkomycin z) in the resulting exconjugants comparing with the parent strain (220 mg l-1 nikkomycin x and 120 mg l-1 nikkomycin z). the exconjugants are genetically stable in the absence of antibiotic resistance selection pressure.conclusiona high nikkomycins producing strain (1100 mg l-1 nikkomycins) was obtained by introduction of an extra nikkomycin biosynthetic gene cluster into the genome of s. ansochromogenes. the strategies presented here could be applicable to other bacteria to improve the yields of secondary metabolites.
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/1475-2859-9-6
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374324
专题	中国科学院大学
通讯作者	Tan,Huarong
作者单位	1.Chinese Academy of Sciences; State Key Laboratory of Microbial Resources, Institute of Microbiology 2.Graduate School of Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Liao,Guojian,Li,Jine,Li,Lei,et al. Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into streptomyces ansochromogenes lead to an improved nikkomycin production[J]. Microbial cell factories,2010,9(1).
APA	Liao,Guojian,Li,Jine,Li,Lei,Yang,Haihua,Tian,Yuqing,&Tan,Huarong.(2010).Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into streptomyces ansochromogenes lead to an improved nikkomycin production.Microbial cell factories,9(1).
MLA	Liao,Guojian,et al."Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into streptomyces ansochromogenes lead to an improved nikkomycin production".Microbial cell factories 9.1(2010).

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