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	Different erythrocyte microrna profiles in low- and high-altitude individuals
	Sun, Liping1; Fan, Fengyan1,2; Li, Ruilin3,4; Niu, Beifang3,4,5; Zhu, Liguo1; Yu, Shuai6; Wang, Shuying1; Li, Cuiying2; Wang, Deqing1
刊名	Frontiers in physiology
	2018-08-14
卷号	9页码:9
关键词	High-altitude hypoxia Tibet Han chinese Tibetan Erythrocyte-derived micrornas
ISSN号	1664-042X
DOI	10.3389/fphys.2018.01099
通讯作者	Li, cuiying(licuiying2013@qq.com) ; Wang, deqing(deqingw@vip.sina.com)
英文摘要	Background: the number of red blood cells (rbcs) increases significantly in response to high-altitude hypoxic environments, and the rbc microrna (mirna) expression pattern is similar to that in whole blood. studies have shown that mirna in plasma can act as a circulating hypoxia-associated marker, but the effect of a high-altitude hypoxic environment on rbc-derived mirnas has not yet been reported. methods: blood samples were collected from 20 han chinese individuals residing at 500m (sichuan han), 10 migrant han chinese citizens residing at 3,658m (tibet han) and 12 native tibetans, and rbc indices measurements and mirna sequencing analyses were performed for the three sample groups. the levels of some markedly altered mirnas at high altitude were subsequently measured from 5 randomly selected samples of each group by real-time pcr. bioinformatic analyses was performed to determine the potential target genes of selected hypoxia-associated mirnas. results: marked changes of several rbc indices were observed among the tibet han population, the tibetan population and the sichuan han population. a total of 516 mirnas derived from rbcs were initially identified by mirna sequencing in the three sample groups. compared with the sichuan han population, 49 mirnas were differentially expressed in the tibet han population (17 upregulated and 32 downregulated). 12 upregulated and 21 downregulated mirnas were observed in the tibetan population compared with the sichuan han population. a total of 40 rbc mirnas were differentially expressed in the tibetan population (15 upregulated and 25 downregulated) compared with the tibet han population. two significantly altered mirnas with the highest expression levels (mirna-144-5p and mir-30b-5p) were selected for real-time pcr analysis, and the results were consistent with those of mirna sequencing. furthermore, bioinformatic analyses showed that some potential target genes of mir-144-5p and mir-30b-5p are involved in the erythroid-hypoxia-, and nitric oxide (no)-related signaling pathways in response to hypoxia. conclusion: our findings provide clear evidence, for the first time, that a high-altitude hypoxic environment significantly affects human rbc mirna profiles.
WOS关键词	RED-BLOOD-CELLS ; PLASMODIUM-FALCIPARUM ; MOUNTAIN-SICKNESS ; IGA NEPHROPATHY ; HYPOXIA ; MIRNAS ; ERYTHROPOIESIS ; ADAPTATION ; EXPRESSION ; MALARIA
WOS研究方向	Physiology
WOS类目	Physiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000441688000002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2374227
专题	计算机网络信息中心
通讯作者	Li, Cuiying; Wang, Deqing
作者单位	1.Chinese Peoples Liberat Army Gen Hosp, Dept Blood Transfus, Beijing, Peoples R China 2.PLA, Dept Blood Transfus, Air Force Gen Hosp, Beijing, Peoples R China 3.Chinese Acad Sci, Comp Network Informat Ctr, Dept High Performance Comp Technol & Applicat Dev, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Guizhou Univ, Sch Med, Guiyang, Guizhou, Peoples R China 6.Peoples Liberat Army Gen Hosp, Clin Lab, Hainan Branch, Sanya, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Sun, Liping,Fan, Fengyan,Li, Ruilin,et al. Different erythrocyte microrna profiles in low- and high-altitude individuals[J]. Frontiers in physiology,2018,9:9.
APA	Sun, Liping.,Fan, Fengyan.,Li, Ruilin.,Niu, Beifang.,Zhu, Liguo.,...&Wang, Deqing.(2018).Different erythrocyte microrna profiles in low- and high-altitude individuals.Frontiers in physiology,9,9.
MLA	Sun, Liping,et al."Different erythrocyte microrna profiles in low- and high-altitude individuals".Frontiers in physiology 9(2018):9.

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