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	Characterization of three autolysins with activity against cereulide-producing bacillus isolates in food matrices
	Geng, Peiling1,2; Hu, Yimin1; Zhou, Guoping3; Yuan, Zhiming1; Hu, Xiaomin1
刊名	International journal of food microbiology
	2017-01-16
卷号	241页码:291-297
关键词	Bacillus cereus Bactericidal enzymes Cocktail Emesis Foodstuff
ISSN号	0168-1605
DOI	10.1016/j.ijfoodmicro.2016.10.030
通讯作者	Yuan, zhiming(yzm@wh.iov.cn) ; Hu, xiaomin(huxm@wh.iov.cn)
英文摘要	Bacillus cereus is a pathogen related with diarrhoeal or emetic food poisoning cases, of which the latter caused by the cereulide-producing isolates are more severe with several reported lethal cases. it is therefore necessary to develop an effective strategy to prevent the propagation of b. cereus in the food supply. in this study, three autolysins from the cereulide-producing b. cereus group isolates, lysis075, lysf8819.1 and ly5cer057, were identified and characterized. the results showed that the three autolysins were highly lytic and bactericidal to the tested cereulide-producing b. cereus group strains and cross-lytic against other tested b. cereus group strains, and they could inhibit the spore germination and propagation of their tested derived emetic strains. physical and chemical characterization showed that all the three autolysins were alkalophilic with the optimal activity at ph 9.0 or 9.5 with one exception of lysf8819.1 also having significant lytic activity at ph 5.0, and they all had relative strong lyric activity at 37-50 degrees c during the 30 minute assay. however, lyscer057 showed relative susceptibility to thermo-condition. remarkably, the separate or cock-tail addition of the three autolysins in food matrices (milk and rice porridge) showed effective bactericidal activity within the tested 2 h. all the results revealed that the three autolysins might be potential candidates to control emetic b. cereus strains in different applications. (c) 2016 published by elsevier b.v.
WOS关键词	COMPLETE GENOME SEQUENCE ; EMETIC TOXIN ; BACTERIOPHAGE ENDOLYSINS ; CATALYTIC DOMAIN ; LIVER-FAILURE ; GROUP STRAINS ; B. CEREUS ; ANTHRACIS ; GENE ; WEIHENSTEPHANENSIS
WOS研究方向	Food Science & Technology ; Microbiology
WOS类目	Food Science & Technology ; Microbiology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000390071600034
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373484
专题	武汉病毒研究所
通讯作者	Yuan, Zhiming; Hu, Xiaomin
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430070, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Wuhan Polytech Univ, Dept Bioengn & Pharmaceut Engn, Wuhan 430023, Peoples R China
推荐引用方式 GB/T 7714	Geng, Peiling,Hu, Yimin,Zhou, Guoping,et al. Characterization of three autolysins with activity against cereulide-producing bacillus isolates in food matrices[J]. International journal of food microbiology,2017,241:291-297.
APA	Geng, Peiling,Hu, Yimin,Zhou, Guoping,Yuan, Zhiming,&Hu, Xiaomin.(2017).Characterization of three autolysins with activity against cereulide-producing bacillus isolates in food matrices.International journal of food microbiology,241,291-297.
MLA	Geng, Peiling,et al."Characterization of three autolysins with activity against cereulide-producing bacillus isolates in food matrices".International journal of food microbiology 241(2017):291-297.

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