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	Quantitative proteomic analysis of mosquito c6/36 cells reveals host proteins involved in zika virus infection
	Xin, Qi-Lin2; Deng, Cheng-Lin2; Chen, Xi3,4; Wang, Jun2; Wang, Shao-Bo2; Wang, Wei1,2; Deng, Fei1,2; Zhang, Bo2; Xiao, Gengfu1,2; Zhang, Lei-Ke1,2
刊名	Journal of virology
	2017-06-01
卷号	91期号:12页码:17
关键词	Zika virus Quantitative proteomics Virus-host interaction Ubiquitin proteasome system Bortezomib
ISSN号	0022-538X
DOI	10.1128/jvi.00554-17
通讯作者	Xiao, gengfu(xiaogf@wh.iov.cn) ; Zhang, lei-ke(zhangleike@wh.iov.cn)
英文摘要	Zika virus (zikv) is an emerging arbovirus belonging to the genus flavivirus of the family flaviviridae. during replication processes, flavivirus manipulates host cell systems to facilitate its replication, while the host cells activate antiviral responses. identification of host proteins involved in the flavivirus replication process may lead to the discovery of antiviral targets. the mosquitoes aedes aegypti and aedes albopictus are epidemiologically important vectors for zikv, and effective restrictions of zikv replication in mosquitoes will be vital in controlling the spread of virus. in this study, an itraq-based quantitative proteomic analysis of zikv-infected aedes albopictus c6/36 cells was performed to investigate host proteins involved in the zikv infection process. a total of 3,544 host proteins were quantified, with 200 being differentially regulated, among which chchd2 can be upregulated by zikv infection in both mosquito c6/36 and human hela cells. our further study indicated that chchd2 can promote zikv replication and inhibit beta interferon (ifn-beta) production in hela cells, suggesting that zikv infection may upregulate chchd2 to inhibit ifn-i production and thus promote virus replication. bioinformatics analysis of regulated host proteins highlighted several zikv infection-regulated biological processes. further study indicated that the ubiquitin proteasome system (ups) plays roles in the zikv entry process and that an fda-approved inhibitor of the 20s proteasome, bortezomib, can inhibit zikv infection in vivo. our study illustrated how host cells respond to zikv infection and also provided a candidate drug for the control of zikv infection in mosquitoes and treatment of zikv infection in patients. importance zikv infection poses great threats to human health, and there is no fda-approved drug available for the treatment of zikv infection. during replication, zikv manipulates host cell systems to facilitate its replication, while host cells activate antiviral responses. identification of host proteins involved in the zikv replication process may lead to the discovery of antiviral targets. in this study, the first quantitative proteomic analysis of zikv-infected cells was performed to investigate host proteins involved in the zikv replication process. bioinformatics analysis highlighted several zikv infection-regulated biological processes. further study indicated that the ubiquitin proteasome system (ups) plays roles in the zikv entry process and that an fda-approved inhibitor of the ups, bortezomib, can inhibit zikv infection in vivo. our study not only illustrated how host cells respond to zikv infection but also provided a candidate drug for the control of zikv infection in mosquitoes and treatment of zikv infection in patients.
WOS关键词	JAPANESE ENCEPHALITIS-VIRUS ; UBIQUITIN-PROTEASOME SYSTEM ; IMMUNE-RESPONSE ; DROSOPHILA ; IDENTIFICATION ; PATHWAY ; ENTRY ; MICE ; CORONAVIRUS ; PERSPECTIVE
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000402169500026
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373430
专题	武汉病毒研究所
通讯作者	Xiao, Gengfu; Zhang, Lei-Ke
作者单位	1.State Key Lab Virol, Wuhan, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China 3.Wuhan Inst Biotechnol, Wuhan, Peoples R China 4.Wuhan Univ, Med Res Inst, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Xin, Qi-Lin,Deng, Cheng-Lin,Chen, Xi,et al. Quantitative proteomic analysis of mosquito c6/36 cells reveals host proteins involved in zika virus infection[J]. Journal of virology,2017,91(12):17.
APA	Xin, Qi-Lin.,Deng, Cheng-Lin.,Chen, Xi.,Wang, Jun.,Wang, Shao-Bo.,...&Zhang, Lei-Ke.(2017).Quantitative proteomic analysis of mosquito c6/36 cells reveals host proteins involved in zika virus infection.Journal of virology,91(12),17.
MLA	Xin, Qi-Lin,et al."Quantitative proteomic analysis of mosquito c6/36 cells reveals host proteins involved in zika virus infection".Journal of virology 91.12(2017):17.

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