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	Linear dna vaccine prepared by large-scale pcr provides protective immunity against h1n1 influenza virus infection in mice
	Wang, Fei1; Chen, Quanjiao2; Li, Shuntang1; Zhang, Chenyao1; Li, Shanshan1; Liu, Min1; Mei, Kun1; Li, Chunhua1; Ma, Lixin1; Yu, Xiaolan1
刊名	Veterinary microbiology
	2017-06-01
卷号	205页码:124-130
关键词	Linear dna vaccine Large-scale pcr Influenza a
ISSN号	0378-1135
DOI	10.1016/j.vetmic.2017.05.015
通讯作者	Ma, lixin(malixing@hubu.edu.cn) ; Yu, xiaolan(yxiaolan@163.com)
英文摘要	Linear dna vaccines provide effective vaccination. however, their application is limited by high cost and small scale of the conventional polymerase chain reaction (pcr) generally used to obtain sufficient amounts of dna effective against epidemic diseases. in this study, a two-step, large-scale pcr was established using a low-cost dna polymerase, rkod, expressed in pichia pastoris. two linear dna vaccines encoding influenza h1n1 hemagglutinin (ha) 1, lec-ha, and pto-lec-ha (with phosphorothioate-modified primers), were produced by the two-step pcr. protective effects of the vaccines were evaluated in a mouse model. balb/c mice were immunized three times with the vaccines or a control dna fragment. all immunized animals were challenged by intranasal administration of a lethal dose of influenza h1n1 virus 2 weeks after the last immunization. sera of the immunized animals were tested for the presence of ha-specific antibodies, and the total ifn-gamma responses induced by linear dna vaccines were measured. the results showed that the dna vaccines but not the control dna induced strong antibody and ifn-gamma responses. additionally, the pto-lec-ha vaccine effectively protected the mice against the lethal homologous mouse-adapted virus, with a survival rate of 100% versus 70% in the lec-ha-vaccinated group, showing that the pto-lec-ha vaccine was more effective than lec-ha. in conclusion, the results indicated that the linear h1n1 ha-coding dna vaccines induced significant immune responses and protected mice against a lethal virus challenge. thus, the low-cost, two-step, large-scale pcr can be considered a potential tool for rapid manufacturing of linear dna vaccines against emerging infectious diseases.
WOS关键词	AVIAN INFLUENZA ; ANTIBODY-RESPONSES ; GENE-TRANSFER ; RABIES VIRUS ; PLASMID DNA ; IN-VIVO ; IMMUNIZATION ; HEMAGGLUTININ ; EXPRESSION ; CHALLENGE
WOS研究方向	Microbiology ; Veterinary Sciences
WOS类目	Microbiology ; Veterinary Sciences
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000404795700022
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373414
专题	武汉病毒研究所
通讯作者	Ma, Lixin; Yu, Xiaolan
作者单位	1.Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Coll Life Sci, Hubei Key Lab Ind Biotechnol, Wuhan 430062, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Fei,Chen, Quanjiao,Li, Shuntang,et al. Linear dna vaccine prepared by large-scale pcr provides protective immunity against h1n1 influenza virus infection in mice[J]. Veterinary microbiology,2017,205:124-130.
APA	Wang, Fei.,Chen, Quanjiao.,Li, Shuntang.,Zhang, Chenyao.,Li, Shanshan.,...&Yu, Xiaolan.(2017).Linear dna vaccine prepared by large-scale pcr provides protective immunity against h1n1 influenza virus infection in mice.Veterinary microbiology,205,124-130.
MLA	Wang, Fei,et al."Linear dna vaccine prepared by large-scale pcr provides protective immunity against h1n1 influenza virus infection in mice".Veterinary microbiology 205(2017):124-130.

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