| Identification and genomic comparison of temperate bacteriophages derived from emetic bacillus cereus | |
| Geng, Peiling1,2; Tian, Shen1; Yuan, Zhiming1; Hu, Xiaomin1 | |
| 刊名 | Plos one
 |
| 2017-09-08 | |
| 卷号 | 12期号:9页码:19 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0184572 |
| 通讯作者 | Yuan, zhiming(yzm@wh.iov.cn) ; Hu, xiaomin(huxm@wh.iov.cn) |
| 英文摘要 | Cereulide-producing bacillus cereus isolates can cause serious emetic ( vomiting) syndrome and even acute lethality. as mobile genetic elements, the exploration of prophages derived from emetic b. cereus isolates will help in our understanding of the genetic diversity and evolution of these pathogens. in this study, five temperate phages derived from cereulide-producing b. cereus strains were induced, with four of them undergoing genomic sequencing. sequencing revealed that they all belong to the siphoviridae family, but presented in different forms in their hosts. pfnc7401 and pfis075 have typical icosahedral heads, probably existing alone as phagemids in the host with self-replicating capability in the lysogenic state. pfefr-4, pfefr-5, and pfatcc7953 have elongated heads, with the genomes of the former two identified as linear dsdna, which could be integrated into the host genome during the lysogenic state. genomic comparison of the four phages with others also derived from emetic b. cereus isolates showed similar genome structures and core genes, thus displaying host spectrum specificity. in addition, phylogenic analysis based on the complete genome and conserved tail fiber proteins of 36 bacillus species-derived phages confirmed that the phages derived from emetic b. cereus strains were highly similar. furthermore, one endolysin lyspfefr-4 was cloned and showed lytic activity against all tested emetic b. cereus strains and cross-lytic activity against some other pathogenic bacteria, implying a potential to control bacterial contamination in the food supply. |
| WOS关键词 | TAIL FIBER GENES ; ESCHERICHIA-COLI ; RNA GENES ; PHAGE ; SEQUENCE ; DNA ; THURINGIENSIS ; PROPHAGE ; ANTHRACIS ; ENDOLYSIN |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| WOS记录号 | WOS:000410001100160 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2373385 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, Zhiming; Hu, Xiaomin |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geng, Peiling,Tian, Shen,Yuan, Zhiming,et al. Identification and genomic comparison of temperate bacteriophages derived from emetic bacillus cereus[J]. Plos one,2017,12(9):19. |
| APA | Geng, Peiling,Tian, Shen,Yuan, Zhiming,&Hu, Xiaomin.(2017).Identification and genomic comparison of temperate bacteriophages derived from emetic bacillus cereus.Plos one,12(9),19. |
| MLA | Geng, Peiling,et al."Identification and genomic comparison of temperate bacteriophages derived from emetic bacillus cereus".Plos one 12.9(2017):19. |
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