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	Structural characterization and analysis of human immunodeficiency virus encapsulating quantum dots
	Yin, Wen1,3; Li, Wei1; Li, Qin1,4; Qin, Chong1,3; Zhang, Zhiping1; Zhang, Xiaowei1; Zhang, Xian-En2; Cui, Zongqiang1
刊名	Journal of nanoscience and nanotechnology
	2018-07-01
卷号	18期号:7页码:4487-4494
关键词	Hiv-1 Quantum dot Structure Negative staining Image
ISSN号	1533-4880
DOI	10.1166/jnn.2018.15347
通讯作者	Zhang, xian-en() ; Cui, zongqiang()
英文摘要	Virus internal labeling by inorganic nanoparticle is a suitable technique for single virus imaging. however, it is unknown that the effect of the internal inorganic nanoparticle on morphology and structure of viral core in mature enveloped human immunodeficiency virus 1 (hiv-1). also, the analysis of intact hiv-1 particles and virus-inorganic nanoparticle hybrids is almost impossible with conventional negative staining because hiv-1 is inherently fragile and unstable. herein, we carried out morphological and structural analysis of a newly constructed quantum dot (qd)-encapsulated infectious hiv-1 particle by using an optimized procedure for the electron microscopic analysis of negatively stained hiv-1 particles. the virus fixation and staining conditions were optimized to ensure the integrity of hiv-1, allowing the ready access to structural information on the viral envelope and core. morphological and structural analysis by optimized tem provide key information about viruses, viral core and nanoparticles, which indicates that the encapsulation of quantum dots had no effect on the morphology or mean size of the viral particle, or on the shape, length, width, or angle of the hiv-1 core.
WOS关键词	ELECTRON-MICROSCOPY ; CRYO-EM ; HIV-1 ; MORPHOLOGY ; PARTICLES ; ENVELOPE ; CORES ; PROTEINS ; TRACKING ; VIRIONS
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
语种	英语
出版者	AMER SCIENTIFIC PUBLISHERS
WOS记录号	WOS:000426057800006
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373339
专题	武汉病毒研究所
通讯作者	Zhang, Xian-En; Cui, Zongqiang
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Biophys, CAS Ctr Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Yin, Wen,Li, Wei,Li, Qin,et al. Structural characterization and analysis of human immunodeficiency virus encapsulating quantum dots[J]. Journal of nanoscience and nanotechnology,2018,18(7):4487-4494.
APA	Yin, Wen.,Li, Wei.,Li, Qin.,Qin, Chong.,Zhang, Zhiping.,...&Cui, Zongqiang.(2018).Structural characterization and analysis of human immunodeficiency virus encapsulating quantum dots.Journal of nanoscience and nanotechnology,18(7),4487-4494.
MLA	Yin, Wen,et al."Structural characterization and analysis of human immunodeficiency virus encapsulating quantum dots".Journal of nanoscience and nanotechnology 18.7(2018):4487-4494.

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