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	Characteristics and optimised fermentation of a novel magnetotactic bacterium, magnetospirillum sp me-1
	Ke, Linfeng1,2; Chen, Yajun1,2; Liu, Pengming1; Liu, Shan1,2; Wu, Dandan1; Yuan, Yihui1; Wu, Yan1; Gao, Meiying1
刊名	Fems microbiology letters
	2018-07-01
卷号	365期号:14页码:9
关键词	Magnetospirillum sp me-1 Isolation Genomic analysis Optimised fermentation Fed-batch cultivation
ISSN号	0378-1097
DOI	10.1093/femsle/fny052
通讯作者	Gao, meiying(mygao@wh.iov.cn)
英文摘要	Magnetotactic bacteria (mtb) can biosynthesise magnetosomes, which have great potential for commercial applications. a new mth strain, magnetospirillum sp. me-1, was isolated and cultivated from freshwater sediments of east lake (wuhan, china) using the limiting dilution method. me-1 had a chain of 17 +/- 4 magnetosomes in the form of cubooctahedral crystals with a shape factor of 0.89. me-1 was closest to magnetospirillum sp. xm-1 according to 16s rrna gene sequence similarity. compared with xm-1, me-1 possessed an additional copy of mampa and a larger mamo in magnetosome-specific genes. me-1 had an intact citric acid cycle, and complete pathway models of ammonium assimilation and dissimilatory nitrate reduction. potential carbon and nitrogen sources in these pathways were confirmed to be used in me-1. adipate was determined to be used in the fermentation medium as a new kind of dicarboxylic acid. the optimised fermentation medium was determined by orthogonal tests. the large-scale production of magnetosomes was achieved and the magnetosome yield (wet weight) reached 120 mg l-1 by fed-batch cultivation of me-1 at 49 h in a 10-l fermenter with the optimised fermentation medium. this study may provide insights into the isolation and cultivation of other new mtb strains and the production of magnetosomes.
WOS关键词	OXYGEN-CONTROLLED FERMENTER ; FED-BATCH CULTURE ; MAGNETOSOME FORMATION ; AQUASPIRILLUM-MAGNETOTACTICUM ; GRYPHISWALDENSE MSR-1 ; MAGNETIC SPIRILLUM ; BETA-OXIDATION ; SP-NOV ; GROWTH ; AMB-1
WOS研究方向	Microbiology
WOS类目	Microbiology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000441114900012
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373250
专题	武汉病毒研究所
通讯作者	Gao, Meiying
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ke, Linfeng,Chen, Yajun,Liu, Pengming,et al. Characteristics and optimised fermentation of a novel magnetotactic bacterium, magnetospirillum sp me-1[J]. Fems microbiology letters,2018,365(14):9.
APA	Ke, Linfeng.,Chen, Yajun.,Liu, Pengming.,Liu, Shan.,Wu, Dandan.,...&Gao, Meiying.(2018).Characteristics and optimised fermentation of a novel magnetotactic bacterium, magnetospirillum sp me-1.Fems microbiology letters,365(14),9.
MLA	Ke, Linfeng,et al."Characteristics and optimised fermentation of a novel magnetotactic bacterium, magnetospirillum sp me-1".Fems microbiology letters 365.14(2018):9.

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