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	Three-fragment fluorescence complementation for imaging of ternary complexes under physiological conditions
	Chen, Minghai1,3; Li, Wei1; Zhang, Zhi-Ping1; Pan, Jingdi1,3; Sun, Yuhan1,3; Zhang, Xiaowei1; Zhang, Xian-En2; Cui, Zongqiang1
刊名	Analytical chemistry
	2018-11-20
卷号	90期号:22页码:13299-13305
ISSN号	0003-2700
DOI	10.1021/acs.analchem.8b02661
通讯作者	Cui, zongqiang(czq@wh.iov.cn)
英文摘要	Protein protein interactions (ppis) occur in a vast variety of cellular processes, and many processes are regulated by multiple protein interactions. identification of ppis is essential for the analysis of biological pathways and to further understand underlying molecular mechanisms. however, visualization and identification of multiprotein complexes, including ternary complexes in living cells under physiological conditions, remains challenging. in this work, we reported a three-fragment fluorescence complementation (tffc) by splitting the venus fluorescent protein for visualizing ternary complexes in living cells under physiological conditions. with this venus-based tffc system, we identified the multi-interaction of weak-affinity ternary complexes under physiological conditions. the tffc system was further applied to the analysis of multi-interactions during the hiv-1 integration process, revealing the important role of the barrier-to-autointegration factor protein in hiv-1 integration. this tffc system provides a useful tool for visualizing and identifying ternary complexes in living cells under physiological conditions.
WOS关键词	LIVING CELLS ; RETROVIRAL DNA ; LEDGF/P75 ; PROTEIN ; BAF ; AUTOINTEGRATION ; VISUALIZATION ; INTEGRATION ; MICROSCOPY
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000451246100025
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373219
专题	武汉病毒研究所
通讯作者	Cui, Zongqiang
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Minghai,Li, Wei,Zhang, Zhi-Ping,et al. Three-fragment fluorescence complementation for imaging of ternary complexes under physiological conditions[J]. Analytical chemistry,2018,90(22):13299-13305.
APA	Chen, Minghai.,Li, Wei.,Zhang, Zhi-Ping.,Pan, Jingdi.,Sun, Yuhan.,...&Cui, Zongqiang.(2018).Three-fragment fluorescence complementation for imaging of ternary complexes under physiological conditions.Analytical chemistry,90(22),13299-13305.
MLA	Chen, Minghai,et al."Three-fragment fluorescence complementation for imaging of ternary complexes under physiological conditions".Analytical chemistry 90.22(2018):13299-13305.

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