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	Crystallographic snapshots of the zika virus ns3 helicase help visualize the reactant water replenishment
	Fang, Junnan1,2; Jing, Xuping3,4; Lu, Guoliang3,4; Xu, Yi1,2; Gong, Peng1,2,3
刊名	Acs infectious diseases
	2019-02-01
卷号	5期号:2页码:177-183
关键词	Flavivirus Zika virus Ns3 helicase Atp hydrolysis Reactant water
ISSN号	2373-8227
DOI	10.1021/acsinfecdis.8b00214
通讯作者	Xu, yi(xuyi70@163.com) ; Gong, peng(gongpeng@wh.iov.cn)
英文摘要	Zika virus (zikv), a positive-strand rna virus belonging to the flavivirus genus, has become an urgent public health concern since recent outbreaks worldwide. its genome replication is facilitated by the viral ns3 protein bearing helicase function. the ns3 helicase uses energy derived from adenosine triphosphate (atp) hydrolysis to unwind rna duplexed regions. structural studies of the flavivirus ns3 helicases have suggested a conserved mechanism of atp hydrolysis. however, the process of the reactant water replenishment, a key part of the hydrolysis cycle, remains elusive. here, we report two high-resolution crystal structures of zikv ns3 helicase in complex with adenosine diphosphate (adp) and mn2+, one with the reactant water already loaded as previously observed and the other with the water molecule still in a loading state. these data suggest that the reactant water replenishment can occur between the release of phosphate and the release of adp and improves the structural basis of the ns3 atp hydrolysis cycle.
WOS关键词	STRUCTURAL BASIS ; RNA ; MECHANISM ; TRANSLOCATION ; SOFTWARE ; INSIGHTS
WOS研究方向	Pharmacology & Pharmacy ; Infectious Diseases
WOS类目	Chemistry, Medicinal ; Infectious Diseases
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000458937100003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373172
专题	武汉病毒研究所
通讯作者	Xu, Yi; Gong, Peng
作者单位	1.Guangzhou Women & Childrens Med Ctr, Guangzhou Inst Pediat, Joint Ctr Translat Precis Med, 318 Renminzhonglu, Guangzhou 510623, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Ctr Translat Precis Med, 44 Xiao Hong Shan, Wuhan 430071, Hubei, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, 44 Xiao Hong Shan, Wuhan 430071, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fang, Junnan,Jing, Xuping,Lu, Guoliang,et al. Crystallographic snapshots of the zika virus ns3 helicase help visualize the reactant water replenishment[J]. Acs infectious diseases,2019,5(2):177-183.
APA	Fang, Junnan,Jing, Xuping,Lu, Guoliang,Xu, Yi,&Gong, Peng.(2019).Crystallographic snapshots of the zika virus ns3 helicase help visualize the reactant water replenishment.Acs infectious diseases,5(2),177-183.
MLA	Fang, Junnan,et al."Crystallographic snapshots of the zika virus ns3 helicase help visualize the reactant water replenishment".Acs infectious diseases 5.2(2019):177-183.

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