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	Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived chlamydomonas reinhardtii
	Yang, Miao1,2,3; Meng, Yingying1,4; Chu, Yadong1; Fan, Yan1,2; Cao, Xupeng1; Xue, Song1; Chi, Zhanyou3
刊名	Biochimica et biophysica acta-molecular and cell biology of lipids
	2018-12-01
卷号	1863期号:12页码:1478-1487
关键词	Chlamydomonas Chloroplast Extra-chloroplastidic tag accumulation Membrane glycerolipids Short-term nitrogen starvation Subcellular glycerolipidomics
ISSN号	1388-1981
DOI	10.1016/j.bbalip.2018.09.009
通讯作者	Xue, song(xuesong@dicp.ac.cn)
英文摘要	In microalgae, triacylglycerol (tag) biosynthesis occurs by parallel pathways involving both the chloroplast and endoplasmic reticulum. a better understanding of contribution of each pathway to tag assembly facilitates enhanced tag production via rational genetic engineering of microalgae. here, using a uplc-ms(/ms) coupled with tlc-gc-based lipidomic platform, the early response of the major glycerolipids to nitrogen stress was analyzed at both the cellular and chloroplastidic levels in the model green alga chlamydomonas reinhardtii. subcellular lipidomic analysis demonstrated that tag was accumulated exclusively outside the chloroplast, and remained unaltered inside the chloroplast after 4 h of nitrogen starvation. this study ascertained the existence of the glycolipid, digalactosyldiacylglycerol (dgdg), outside the chloroplast and the betaine lipid, diacylglycerol-n,n,n-trimethylhomoserine (dgts), inside the chloroplast. the newly synthesized dgdg and dgts prominently increased at the extra-chloroplastidic compartments and served as the major precursors for tag biosynthesis. in particular, dgdg contributed to the extra-chloroplastidic tag assembly in form of diacylglycerol (dag) and dgts in form of acyl groups. the chloroplastidic membrane lipid, monogalactosyldiacylglycerol (mgdg), was proposed to primarily offer dag for tag formation outside the chloroplast. this study provides valuable insights into the subcellular glycerolipidomics and unveils the acyl flux into the extra-chloroplastidic tag in microalgae.
WOS关键词	ACID BETA-OXIDATION ; LIPID-METABOLISM ; NANNOCHLOROPSIS-OCEANICA ; MEMBRANE PHOSPHOLIPIDS ; PHOSPHATE DEPRIVATION ; PLASMA-MEMBRANE ; BIOSYNTHESIS ; MICROALGAE ; ACYLTRANSFERASE ; IDENTIFICATION
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000449898800005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373072
专题	大连化学物理研究所
通讯作者	Xue, Song
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 4.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Yang, Miao,Meng, Yingying,Chu, Yadong,et al. Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived chlamydomonas reinhardtii[J]. Biochimica et biophysica acta-molecular and cell biology of lipids,2018,1863(12):1478-1487.
APA	Yang, Miao.,Meng, Yingying.,Chu, Yadong.,Fan, Yan.,Cao, Xupeng.,...&Chi, Zhanyou.(2018).Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived chlamydomonas reinhardtii.Biochimica et biophysica acta-molecular and cell biology of lipids,1863(12),1478-1487.
MLA	Yang, Miao,et al."Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived chlamydomonas reinhardtii".Biochimica et biophysica acta-molecular and cell biology of lipids 1863.12(2018):1478-1487.

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