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	Identification of peptide profiling of deer antler by reversed phase liquid chromatography-tandem mass spectrometry and bioactivities in simulated gastrointestinal digestion
	Yu Yang; Yan Jia-Ze; Jin Yan
刊名	Chinese journal of analytical chemistry
	2018-04-01
卷号	46期号:4页码:601-608
关键词	Deer antler Simulated gastrointestinal digestion Reversed phase liquid chromatography-tandem mass spectrometry Peptide profiling Biological activities
ISSN号	0253-3820
DOI	10.11895/j.issn.0253.3820.171421
通讯作者	Jin yan(yanjin@dicp.ac.cn)
英文摘要	Reversed phase liquid chromatography-tandem mass spectrometry (rplc-ms/ms) was utilized to investigate peptide profiling and bioactivities of antler aqueous extract (aae), digested antler aqueous extract (aed) and powder (pd). a total of 23, 417 and 389 peptides, as well as 15, 146 and 75 collagen peptides were identified from aae, aed and pd, respectively. angiotensin converting enzyme (ace) inhibitory activity, dipeptidyl peptidase iv (dpp-iv) inhibitory activity, prolyl endopeptidase (pep) inhibitory activity and antioxidant activity were used to evaluate the bioactivities of aae, aed and pd, and it was found that the sequence of their bioactivities was aae < aed < pd. all the results above proved that aed released more collagen peptides and pd possessed better biological activities. it suggests that the two edible ways of antler are complemented each other and have their own advantages.
WOS关键词	VELVET ANTLER ; ANTIOXIDANT ACTIVITY ; PROLYL OLIGOPEPTIDASE ; INHIBITORY PEPTIDES ; PROTEOME ANALYSIS ; CERVUS-ELAPHUS ; IN-VITRO ; YOGURT ; POLYPEPTIDE ; EXTRACT
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000455897800011
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372908
专题	大连化学物理研究所
通讯作者	Jin Yan
作者单位	Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem & Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Yu Yang,Yan Jia-Ze,Jin Yan. Identification of peptide profiling of deer antler by reversed phase liquid chromatography-tandem mass spectrometry and bioactivities in simulated gastrointestinal digestion[J]. Chinese journal of analytical chemistry,2018,46(4):601-608.
APA	Yu Yang,Yan Jia-Ze,&Jin Yan.(2018).Identification of peptide profiling of deer antler by reversed phase liquid chromatography-tandem mass spectrometry and bioactivities in simulated gastrointestinal digestion.Chinese journal of analytical chemistry,46(4),601-608.
MLA	Yu Yang,et al."Identification of peptide profiling of deer antler by reversed phase liquid chromatography-tandem mass spectrometry and bioactivities in simulated gastrointestinal digestion".Chinese journal of analytical chemistry 46.4(2018):601-608.

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