QQ客服 官方微博 反馈留言

	Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
	Liu, Shiyue1,2,3; Liu, Ya-Jun1,2; Feng, Yingang1,2; Li, Bin1,2; Cui, Qiu1,2
刊名	Biotechnology for biofuels
	2019-02-18
卷号	12页码:12
关键词	Cellulase Cellulosome Clostridium thermocellum Fermentable sugar Lignocellulose Saccharification
ISSN号	1754-6834
DOI	10.1186/s13068-019-1374-2
通讯作者	Cui, qiu(cuiqiu@qibebt.ac.cn)
英文摘要	Backgroundthe industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. consolidated bio-saccharification (cbs) is considered a promising process for lignocellulose solubilization depending on whole-cell biocatalysts that simultaneously perform effective cellulase production and hydrolysis. however, it usually takes a long time to reach a high saccharification level using the current cbs biocatalyst and process.resultsto promote the saccharification efficiency and reduce the cost, a clostridium thermocellum recombinant strain pyrf::kbm was constructed as a new cbs biocatalyst in this study. the key cbs factors, including the medium, inoculum size and cultivation, and substrate load, were investigated and optimized. the saccharification process was also stimulated by adding free hemicellulases, suggesting the need to further enhance hemicellulase activity of the whole-cell catalyst. under the optimal conditions, the cbs process was shortened by 50% with pretreated wheat straw as the substrate. the sugar yield reached 0.795g/g and the saccharification level was 89.3%.conclusionsthis work provided a new biocatalyst and an optimized process of cbs and confirmed that cbs is a feasible strategy for cost-efficient solubilization of lignocellulose, which will greatly promote the industrial utilization of lignocellulosic biomass.
WOS关键词	CLOSTRIDIUM-THERMOCELLUM ; ETHANOL ; CELLULOSE ; GLUCOSE ; BIOMASS ; GROWTH ; YIELD
WOS研究方向	Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	BMC
WOS记录号	WOS:000459152400002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372764
专题	大连化学物理研究所
通讯作者	Cui, Qiu
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Dalian Natl Lab Clean Energy, Dalian, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Shiyue,Liu, Ya-Jun,Feng, Yingang,et al. Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization[J]. Biotechnology for biofuels,2019,12:12.
APA	Liu, Shiyue,Liu, Ya-Jun,Feng, Yingang,Li, Bin,&Cui, Qiu.(2019).Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization.Biotechnology for biofuels,12,12.
MLA	Liu, Shiyue,et al."Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization".Biotechnology for biofuels 12(2019):12.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。