Structural studies on a novel fucogalactan sulfate extracted from the brown seaweed Laminaria japonica

doi:10.1016/j.ijbiomac.2010.05.010

	Structural studies on a novel fucogalactan sulfate extracted from the brown seaweed Laminaria japonica
	Wang, Jing1,2 ; Zhang, Quanbin 1; Zhang, Zhongshan 1,2; Zhang, Hong 1; Niu, Xizhen 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2010-08-01
卷号	47 期号:2 页码:126-131
关键词	Fucogalactan Sulfate Structure Brown Algae Nmr Laminaria Japonica
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2010.05.010
文献子类	Article
英文摘要	A low molecular weight fucogalactan, obtained from the brown seaweed Laminaria japonica, was separated into three fractions (LF1, LF2 and LF3) by DEAE-Sepharose FF column chromatography. All three fractions contained predominantly fucose, sulfate group and galactose. The results showed that the main fraction LF2 consisted of L-fucose, D-galactose and sulfate at a molar ratio 6:1:9. Structural study on the LF2 was carried out by NMR spectroscopy. The backbone of LF2 was primarily (1 -> 3)-linked alpha-L-fucopyranose residues (75%) and a few (1 -> 4)-alpha-L-fucopyranose linkages (25%). The branch points were at C-4 of 3-linked alpha-L-fucopyranose residues by beta-D-galactopyranose unites (35%, molar ratio) or at C-2 of 3-linked alpha-L-fucopyranose residues by non-reducing terminal fucose unites (65%, molar ratio). Sulfate groups occupied at position C-4 or C-2, sometimes C-2, 4 to fucose residues, and C-3 and/or C-4 to galactose residues. The structure of LF2 was supposed as following:; A low molecular weight fucogalactan, obtained from the brown seaweed Laminaria japonica, was separated into three fractions (LF1, LF2 and LF3) by DEAE-Sepharose FF column chromatography. All three fractions contained predominantly fucose, sulfate group and galactose. The results showed that the main fraction LF2 consisted of L-fucose, D-galactose and sulfate at a molar ratio 6:1:9. Structural study on the LF2 was carried out by NMR spectroscopy. The backbone of LF2 was primarily (1 -> 3)-linked alpha-L-fucopyranose residues (75%) and a few (1 -> 4)-alpha-L-fucopyranose linkages (25%). The branch points were at C-4 of 3-linked alpha-L-fucopyranose residues by beta-D-galactopyranose unites (35%, molar ratio) or at C-2 of 3-linked alpha-L-fucopyranose residues by non-reducing terminal fucose unites (65%, molar ratio). Sulfate groups occupied at position C-4 or C-2, sometimes C-2, 4 to fucose residues, and C-3 and/or C-4 to galactose residues. The structure of LF2 was supposed as following: [GRAPHICS] (C) 2010 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000279999200008
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/6161]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jing,Zhang, Quanbin,Zhang, Zhongshan,et al. Structural studies on a novel fucogalactan sulfate extracted from the brown seaweed Laminaria japonica[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2010,47(2):126-131.
APA	Wang, Jing,Zhang, Quanbin,Zhang, Zhongshan,Zhang, Hong,&Niu, Xizhen.(2010).Structural studies on a novel fucogalactan sulfate extracted from the brown seaweed Laminaria japonica.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,47(2),126-131.
MLA	Wang, Jing,et al."Structural studies on a novel fucogalactan sulfate extracted from the brown seaweed Laminaria japonica".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 47.2(2010):126-131.