Cloning and expression analysis of a HSP70 gene from Pacific abalone (Haliotis discus hannai)

doi:10.1016/j.fsi.2006.03.014

	Cloning and expression analysis of a HSP70 gene from Pacific abalone (Haliotis discus hannai)
	Cheng, Peizhou ; Liu, Xiao ; Zhang, Guofan ; He, Jianguo
刊名	FISH & SHELLFISH IMMUNOLOGY
	2007
卷号	22 期号:1-2 页码:77-87
关键词	Haliotis Discus Hannai Hsp70 Expression Heat Shock Vibrio Anguillarum
ISSN号	1050-4648
DOI	10.1016/j.fsi.2006.03.014
文献子类	Article
英文摘要	Heat shock protein 70 (HSP70), the primary member of HSPs that are responsive of thermal stress, is found in all multicellular organisms and functions mostly as molecular chaperon. The inducible HSP70 cDNA cloned from Pacific abalone (Haliotis discus hannai) using rapid amplification of cDNA ends (RACE), was highly homologous to other HSP70 genes. The full-length cDNA of the Pacific abalone HSP70 was 2631 bp, consisting of a 5'-terminal untranslated region (UTR) of 90 bp, a 3'-terminal UTR of 573 by with a canonical polyadenylation signal sequence AATAAA and a poly (A) tail, and an open reading frame of 1968 bp. The HSP70 cDNA encoded a polypeptide of 655 amino acids with an ATPase domain of 382 amino acids, the substrate peptide binding domain of 161 amino acids and a C-terminus domain of 112 amino acids. The temporal expression of HSP70 was measured by semi-quantitative RT-PCR after heat shock and bacterial challenge. Challenge of Pacific abalone with heat shock or the pathogenic bacteria Vibrio anguillarum resulted in a dramatic increase in the expression of HSP70 mRNA level in muscle, followed by a recovery to normal level after 96 h. Unlike the muscle, the levels of HSP70 expression in gills reached the top at 12 h and maintained a relatively high level compared with the control after thermal and bacterial challenge. The upregulated mRNA expression of HSP70 in the abalone following heat shock and infection response indicates that the HSP70 gene is inducible and involved in immune response. (c) 2006 Elsevier Ltd. All rights reserved.; Heat shock protein 70 (HSP70), the primary member of HSPs that are responsive of thermal stress, is found in all multicellular organisms and functions mostly as molecular chaperon. The inducible HSP70 cDNA cloned from Pacific abalone (Haliotis discus hannai) using rapid amplification of cDNA ends (RACE), was highly homologous to other HSP70 genes. The full-length cDNA of the Pacific abalone HSP70 was 2631 bp, consisting of a 5'-terminal untranslated region (UTR) of 90 bp, a 3'-terminal UTR of 573 by with a canonical polyadenylation signal sequence AATAAA and a poly (A) tail, and an open reading frame of 1968 bp. The HSP70 cDNA encoded a polypeptide of 655 amino acids with an ATPase domain of 382 amino acids, the substrate peptide binding domain of 161 amino acids and a C-terminus domain of 112 amino acids. The temporal expression of HSP70 was measured by semi-quantitative RT-PCR after heat shock and bacterial challenge. Challenge of Pacific abalone with heat shock or the pathogenic bacteria Vibrio anguillarum resulted in a dramatic increase in the expression of HSP70 mRNA level in muscle, followed by a recovery to normal level after 96 h. Unlike the muscle, the levels of HSP70 expression in gills reached the top at 12 h and maintained a relatively high level compared with the control after thermal and bacterial challenge. The upregulated mRNA expression of HSP70 in the abalone following heat shock and infection response indicates that the HSP70 gene is inducible and involved in immune response. (c) 2006 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识	查看原文
语种	英语
WOS记录号	WOS:000241924000008
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/6035]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Peizhou,Liu, Xiao,Zhang, Guofan,et al. Cloning and expression analysis of a HSP70 gene from Pacific abalone (Haliotis discus hannai)[J]. FISH & SHELLFISH IMMUNOLOGY,2007,22(1-2):77-87.
APA	Cheng, Peizhou,Liu, Xiao,Zhang, Guofan,&He, Jianguo.(2007).Cloning and expression analysis of a HSP70 gene from Pacific abalone (Haliotis discus hannai).FISH & SHELLFISH IMMUNOLOGY,22(1-2),77-87.
MLA	Cheng, Peizhou,et al."Cloning and expression analysis of a HSP70 gene from Pacific abalone (Haliotis discus hannai)".FISH & SHELLFISH IMMUNOLOGY 22.1-2(2007):77-87.