Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway

doi:10.1016/j.dci.2017.02.004

	Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway
	Tang, Xueying 1,2,3,5; Huang, Baoyu1,3,5 ; Zhang, Linlin 1,3,5; Li, Li 1,4,5; Zhang, Guofan 1,3,5
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2017-07-01
卷号	72 页码:21-29
关键词	Crassostrea Gigas Irak4 Myd88 Tlr Nf-kappa b
DOI	10.1016/j.dci.2017.02.004
文献子类	Article
英文摘要	Interleukin-1 receptor-associated kinases (IRAKs) play important roles in MyD88-dependent TLR signaling, the crucial innate immune pathway in molluscs. In this study, we examined the full-length IRAK4 genetic sequence in the Pacific oyster (Crassostrea gigas) by molecular cloning. Phylogenetic analysis revealed that CgIRAK4 is most closely related to Mytilus edulis, and forms a Glade with other molluscs. CgIRAK4 transcripts are widely expressed in all tissues, with the highest expression observed in the hemocytes and gill. Moreover, CgIRAK4 is significantly upregulated after Oyster herpesvirus-1 microvariant (0sHV-1 mu var), Vibrio alginolyticus, and poly I:C challenge. Yeast two-hybrid and co-immunoprecipitation assays reveal that the CgIRAK4 death domain is necessary to mediate interaction between CgIRAK4 and two CgMyD88 isoforms. In addition, CgIRAK4 overexpression cannot induce NF-kappa B transcriptional activity, but blocks that induced by CgMyD88 in HEK293T cells. These findings elucidate the mechanisms of MyD88-dependent TLR signaling in molluscs, and the differences in IRAK-mediated pathway activation between invertebrates and vertebrates. (C) 2017 Elsevier Ltd. All rights reserved.
语种	英语
WOS记录号	WOS:000400038100003
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/137057]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China 5.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Tang, Xueying,Huang, Baoyu,Zhang, Linlin,et al. Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,72:21-29.
APA	Tang, Xueying,Huang, Baoyu,Zhang, Linlin,Li, Li,&Zhang, Guofan.(2017).Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,72,21-29.
MLA	Tang, Xueying,et al."Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 72(2017):21-29.