Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein

doi:10.1016/j.fsi.2010.10.008

	Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein
	Ding, Jianfeng 1,2,3; Li, Juan 1,2; Bao, Yongbo 1,2; Li, Li 1; Wu, Fucun 1; Zhang, Guofan 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2011
卷号	30 期号:1 页码:163-172
关键词	Haliotis Discus Hannai Ino Chicken-type Lysozyme Promoter Recombinant Protein Bacteriolytic Activity
ISSN号	1050-4648
DOI	10.1016/j.fsi.2010.10.008
文献子类	Article
英文摘要	A chicken-type lysozyme was obtained and characterized from the abalone Haliotis discus hannai In (HdLysC). The full-length cDNA of HdLysC was 586 bp, and it contained an open reading frame of 441 bp, encoding a 147-amino acid protein with a calculated molecular mass of 15.64 kDa and an isoelectric point of 4.87. The amino acid sequence of HdLysC possessed all conserved features critical for the fundamental structure and function of c-type lysozymes, including the two catalytic residues, Glu54 and Asp70. The genomic length of HdLysC was 2865 bp, with four exons interrupted by three introns. The genomic structure of HdLysC was more similar to vertebrates than invertebrates. Many putative transcription factor binding sites involved in the immune system and cancer were found in the promoter region of HdLysC. Quantitative real-time RT-PCR detected HdLysC expression in all examined tissues, as well as in an expression profile of abalone gills challenged with bacteria Vibrio anguillarum. HdLysC transcripts were found to be most abundantly expressed in mantles, and weakly expressed in hemocytes, and increased expression of HdLysC also observed after bacterial stimulation. HdLysC was expressed in Escherichia coli, and the recombinant protein showed bacteriolytic activity against both Gram-positive and Gram-negative bacteria. Of the Vibrio species tested, more effective activity was detected against V. anguillarum. (C) 2010 Elsevier Ltd. All rights reserved.; A chicken-type lysozyme was obtained and characterized from the abalone Haliotis discus hannai In (HdLysC). The full-length cDNA of HdLysC was 586 bp, and it contained an open reading frame of 441 bp, encoding a 147-amino acid protein with a calculated molecular mass of 15.64 kDa and an isoelectric point of 4.87. The amino acid sequence of HdLysC possessed all conserved features critical for the fundamental structure and function of c-type lysozymes, including the two catalytic residues, Glu54 and Asp70. The genomic length of HdLysC was 2865 bp, with four exons interrupted by three introns. The genomic structure of HdLysC was more similar to vertebrates than invertebrates. Many putative transcription factor binding sites involved in the immune system and cancer were found in the promoter region of HdLysC. Quantitative real-time RT-PCR detected HdLysC expression in all examined tissues, as well as in an expression profile of abalone gills challenged with bacteria Vibrio anguillarum. HdLysC transcripts were found to be most abundantly expressed in mantles, and weakly expressed in hemocytes, and increased expression of HdLysC also observed after bacterial stimulation. HdLysC was expressed in Escherichia coli, and the recombinant protein showed bacteriolytic activity against both Gram-positive and Gram-negative bacteria. Of the Vibrio species tested, more effective activity was detected against V. anguillarum. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识	查看原文
语种	英语
WOS记录号	WOS:000286905800021
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12028]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Dalian Ocean Univ, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ding, Jianfeng,Li, Juan,Bao, Yongbo,et al. Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):163-172.
APA	Ding, Jianfeng,Li, Juan,Bao, Yongbo,Li, Li,Wu, Fucun,&Zhang, Guofan.(2011).Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein.FISH & SHELLFISH IMMUNOLOGY,30(1),163-172.
MLA	Ding, Jianfeng,et al."Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):163-172.