Gene discovery, comparative analysis and expression profile reveal the complexity of the Crassostrea gigas apoptosis system

doi:10.1016/j.dci.2011.01.005

	Gene discovery, comparative analysis and expression profile reveal the complexity of the Crassostrea gigas apoptosis system
	Zhang, Linlin 1,2; Li, Li 1; Zhang, Guofan 1
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2011-05-01
卷号	35 期号:5 页码:603-610
关键词	Lophotrochozoa Apoptosis Evolution Crassostrea Gigas Bir Immune Response
ISSN号	0145-305X
DOI	10.1016/j.dci.2011.01.005
文献子类	Article
英文摘要	Apoptosis system was reported to play important role in organism immunity, but it was a currently understudied respect in molluscan immunity studies. Base on the recent generation of ESTs in the pacific oyster. Crassostrea gigas, a survey of apoptosis-related molecules was conducted in the assembled uni-genes, we found that the basic genes and domains in apoptosis-associated proteins were conserved, the overall apoptotic machinery was complex in C. gigas and that the organism had an expanded number of putative baculovirus inhibitor of apoptosis repeat domains. Moreover, four typical apoptosis-related genes were cloned in C. gigas and compared with the sequences of these genes in Drosophila melanogaster and Homo sapiens. The expression level of these four apoptosis-related genes in the hemolymph increased dramatically in the presence of the bacteria, Vibrio anguillarum, indicating their role in bacterial defense. Our results suggest that the oyster apoptosis system is not simple and cannot be represented by model invertebrates. (C) 2011 Elsevier Ltd. All rights reserved.; Apoptosis system was reported to play important role in organism immunity, but it was a currently understudied respect in molluscan immunity studies. Base on the recent generation of ESTs in the pacific oyster. Crassostrea gigas, a survey of apoptosis-related molecules was conducted in the assembled uni-genes, we found that the basic genes and domains in apoptosis-associated proteins were conserved, the overall apoptotic machinery was complex in C. gigas and that the organism had an expanded number of putative baculovirus inhibitor of apoptosis repeat domains. Moreover, four typical apoptosis-related genes were cloned in C. gigas and compared with the sequences of these genes in Drosophila melanogaster and Homo sapiens. The expression level of these four apoptosis-related genes in the hemolymph increased dramatically in the presence of the bacteria, Vibrio anguillarum, indicating their role in bacterial defense. Our results suggest that the oyster apoptosis system is not simple and cannot be represented by model invertebrates. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题	Immunology ; Zoology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000289333500011
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12020]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Linlin,Li, Li,Zhang, Guofan. Gene discovery, comparative analysis and expression profile reveal the complexity of the Crassostrea gigas apoptosis system[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(5):603-610.
APA	Zhang, Linlin,Li, Li,&Zhang, Guofan.(2011).Gene discovery, comparative analysis and expression profile reveal the complexity of the Crassostrea gigas apoptosis system.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,35(5),603-610.
MLA	Zhang, Linlin,et al."Gene discovery, comparative analysis and expression profile reveal the complexity of the Crassostrea gigas apoptosis system".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 35.5(2011):603-610.