Temporal distribution of the archaeal community in the Changjiang Estuary hypoxia area and the adjacent East China Sea as determined by denaturing gradient gel electrophoresis and multivariate analysis

doi:10.1139/W11-037

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	Temporal distribution of the archaeal community in the Changjiang Estuary hypoxia area and the adjacent East China Sea as determined by denaturing gradient gel electrophoresis and multivariate analysis
	Liu, Min 1,2; Xiao, Tian 1; Wu, Ying 3; Zhou, Feng 4; Zhang, Wuchang 1
刊名	CANADIAN JOURNAL OF MICROBIOLOGY
	2011-06-01
卷号	57 期号:6 页码:504-513
关键词	Archaeal Community Structure Denaturing Gradient Gel Electrophoresis Canonical Correspondence Analysis Changjiang Estuary Hypoxia Area East China Sea
ISSN号	0008-4166
DOI	10.1139/W11-037
文献子类	Article
英文摘要	The archaeal community and the effects of environmental factors on microbial community distribution were investigated at five sampling sites in the Changjiang Estuary hypoxia area and the adjacent East China Sea in June, August, and October 2006. Profiles of the archaeal communities were generated by denaturing gradient gel electrophoresis of 16S rRNA genes followed by DNA sequence analysis, and the results were analyzed by multivariate statistical analysis. Denaturing gradient gel electrophoresis band patterns were analyzed by cluster analysis to assess temporal changes in the genetic diversity of the archaeal communities. Most of the October samples grouped together separately from those of June and August. Analysis of DNA sequences revealed that the dominant archaeal groups in the Changjiang Estuary hypoxia area and the adjacent East China Sea were affiliated with Euryarchaeota (mainly marine group II) and Crenarchaeota. The effects of environmental factors on the archaeal community distribution were analyzed by the ordination technique of canonical correspondence analysis. Salinity had a significant effect on the archaeal community composition.; The archaeal community and the effects of environmental factors on microbial community distribution were investigated at five sampling sites in the Changjiang Estuary hypoxia area and the adjacent East China Sea in June, August, and October 2006. Profiles of the archaeal communities were generated by denaturing gradient gel electrophoresis of 16S rRNA genes followed by DNA sequence analysis, and the results were analyzed by multivariate statistical analysis. Denaturing gradient gel electrophoresis band patterns were analyzed by cluster analysis to assess temporal changes in the genetic diversity of the archaeal communities. Most of the October samples grouped together separately from those of June and August. Analysis of DNA sequences revealed that the dominant archaeal groups in the Changjiang Estuary hypoxia area and the adjacent East China Sea were affiliated with Euryarchaeota (mainly marine group II) and Crenarchaeota. The effects of environmental factors on the archaeal community distribution were analyzed by the ordination technique of canonical correspondence analysis. Salinity had a significant effect on the archaeal community composition.
学科主题	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000291449800007
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11712]
专题	海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100086, Peoples R China 3.E China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200241, Peoples R China 4.State Ocean Adm, Inst Oceanog 2, State Key Lab Satellite Ocean Environm Dynam, Hangzhou 310012, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Min,Xiao, Tian,Wu, Ying,et al. Temporal distribution of the archaeal community in the Changjiang Estuary hypoxia area and the adjacent East China Sea as determined by denaturing gradient gel electrophoresis and multivariate analysis[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2011,57(6):504-513.
APA	Liu, Min,Xiao, Tian,Wu, Ying,Zhou, Feng,&Zhang, Wuchang.(2011).Temporal distribution of the archaeal community in the Changjiang Estuary hypoxia area and the adjacent East China Sea as determined by denaturing gradient gel electrophoresis and multivariate analysis.CANADIAN JOURNAL OF MICROBIOLOGY,57(6),504-513.
MLA	Liu, Min,et al."Temporal distribution of the archaeal community in the Changjiang Estuary hypoxia area and the adjacent East China Sea as determined by denaturing gradient gel electrophoresis and multivariate analysis".CANADIAN JOURNAL OF MICROBIOLOGY 57.6(2011):504-513.