Enhanced immunostimulatory and antitumor activity of different derivatives of kappa-carrageenan oligosaccharides from Kappaphycus striatum

doi:10.1007/s10811-010-9536-4

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	Enhanced immunostimulatory and antitumor activity of different derivatives of kappa-carrageenan oligosaccharides from Kappaphycus striatum
	Yuan, Huamao; Song, Jinming; Li, Xuegang; Li, Ning; Liu, Song
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2011-02-01
卷号	23 期号:1 页码:59-65
关键词	Carrageenan Oligosaccharides Derivatives Antitumor Immunomodulation Chemical Modification
ISSN号	0921-8971
DOI	10.1007/s10811-010-9536-4
文献子类	Article
英文摘要	Chemical modification of carbohydrates can lead to differences in their biological activities. We previously showed that kappa-carrageenan oligosaccharides from Kappaphycus striatum have antitumor and immunomodulation effects on S180-bearing mice. In this study, we tested the hypothesis that different chemical modifications of carrageenan oligosaccharides enhance their activities. The mice inoculated with S180 cell suspension were treated p.o. with carrageenan oligosaccharides and their sulfated, acetylated, and phosphorylated derivatives (50, 100, and 200 mu g g(-1)) for 14 days. Transplantable tumor inhibition rate and macrophage phagocytosis, quantitative hemolysis of sheep red blood cells, lymphocyte proliferation, the activity of natural killer cells, production of interleukin-2, and tumor necrosis factor-alpha were also analyzed. As expected, treatment with different kappa-carrageenan oligosaccharides derivatives resulted in an increase in tumor inhibition rate and macrophage phagocytosis and cellular immunity, especially on spleen lymphocyte proliferation. The sulfated derivative at the dose 200 mu g g(-1) per day showed the highest antitumor activity with the 54.12% tumor weight inhibition and elicited an increase in nature killer cells activity up to 76.1% on S180-bearing mice, which were both significantly higher than the unmodified oligosaccharides. It suggested that chemical modification (especially sulfation) of carrageenan oligosaccharides can enhance their antitumor effect and boost their antitumor immunity.; Chemical modification of carbohydrates can lead to differences in their biological activities. We previously showed that kappa-carrageenan oligosaccharides from Kappaphycus striatum have antitumor and immunomodulation effects on S180-bearing mice. In this study, we tested the hypothesis that different chemical modifications of carrageenan oligosaccharides enhance their activities. The mice inoculated with S180 cell suspension were treated p.o. with carrageenan oligosaccharides and their sulfated, acetylated, and phosphorylated derivatives (50, 100, and 200 mu g g(-1)) for 14 days. Transplantable tumor inhibition rate and macrophage phagocytosis, quantitative hemolysis of sheep red blood cells, lymphocyte proliferation, the activity of natural killer cells, production of interleukin-2, and tumor necrosis factor-alpha were also analyzed. As expected, treatment with different kappa-carrageenan oligosaccharides derivatives resulted in an increase in tumor inhibition rate and macrophage phagocytosis and cellular immunity, especially on spleen lymphocyte proliferation. The sulfated derivative at the dose 200 mu g g(-1) per day showed the highest antitumor activity with the 54.12% tumor weight inhibition and elicited an increase in nature killer cells activity up to 76.1% on S180-bearing mice, which were both significantly higher than the unmodified oligosaccharides. It suggested that chemical modification (especially sulfation) of carrageenan oligosaccharides can enhance their antitumor effect and boost their antitumor immunity.
学科主题	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000287330400008
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11666]
专题	海洋研究所_海洋生态与环境科学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Huamao,Song, Jinming,Li, Xuegang,et al. Enhanced immunostimulatory and antitumor activity of different derivatives of kappa-carrageenan oligosaccharides from Kappaphycus striatum[J]. JOURNAL OF APPLIED PHYCOLOGY,2011,23(1):59-65.
APA	Yuan, Huamao,Song, Jinming,Li, Xuegang,Li, Ning,&Liu, Song.(2011).Enhanced immunostimulatory and antitumor activity of different derivatives of kappa-carrageenan oligosaccharides from Kappaphycus striatum.JOURNAL OF APPLIED PHYCOLOGY,23(1),59-65.
MLA	Yuan, Huamao,et al."Enhanced immunostimulatory and antitumor activity of different derivatives of kappa-carrageenan oligosaccharides from Kappaphycus striatum".JOURNAL OF APPLIED PHYCOLOGY 23.1(2011):59-65.