Isolation and identification of bacteria associated with the surfaces of several algal species

doi:10.1007/s00343-009-9165-4

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	Isolation and identification of bacteria associated with the surfaces of several algal species
	Wang Zifeng 1,2; Xiao Tian 1; Pang Shaojun 1; Liu Min 1; Yue Haidong 1
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2009-08-01
卷号	27 期号:3 页码:487-492
关键词	Epiphytic Bacteria Algae 16s Rrna Gene Phylogeny
ISSN号	0254-4059
DOI	10.1007/s00343-009-9165-4
文献子类	Article
英文摘要	We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses. Twelve strains of bacteria were obtained from the surfaces of the following four species of algae: Gracilaria textorii, Ulva pertusa, Laminaria japonica, and Polysiphonia urceolata. The isolated strains of bacteria can be divided into two groups: Halomonas and Vibrio, in physiology, biochemical characteristics and 16S rDNA sequence analyses. The phylogenetic tree constructed based on 16S rDNA sequences of the isolates shows four obvious clusters, Halomonas venusta, Vibrio tasmaniensis, Vibrio lentus, and Vibrio splendidus. Isolates from the surface of P. urceolata are more abundant and diverse, of which strains P9 and P28 have a 16S rDNA sequence very similar (97.5%-99.8%) to that of V. splendidus. On the contrary, the isolates from the surfaces of G textorii, U. pertusa and L. japonica are quite simple and distribute on different branches of the phylogenetic tree. In overall, the results of this study indicate that the genetic relationships among the isolates are quite close and display a certain level of host species specificity, and alga-associated bacteria species are algal species specific.; We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses. Twelve strains of bacteria were obtained from the surfaces of the following four species of algae: Gracilaria textorii, Ulva pertusa, Laminaria japonica, and Polysiphonia urceolata. The isolated strains of bacteria can be divided into two groups: Halomonas and Vibrio, in physiology, biochemical characteristics and 16S rDNA sequence analyses. The phylogenetic tree constructed based on 16S rDNA sequences of the isolates shows four obvious clusters, Halomonas venusta, Vibrio tasmaniensis, Vibrio lentus, and Vibrio splendidus. Isolates from the surface of P. urceolata are more abundant and diverse, of which strains P9 and P28 have a 16S rDNA sequence very similar (97.5%-99.8%) to that of V. splendidus. On the contrary, the isolates from the surfaces of G textorii, U. pertusa and L. japonica are quite simple and distribute on different branches of the phylogenetic tree. In overall, the results of this study indicate that the genetic relationships among the isolates are quite close and display a certain level of host species specificity, and alga-associated bacteria species are algal species specific.
学科主题	Limnology ; Oceanography
URL标识	查看原文
语种	英语
WOS记录号	WOS:000270742000009
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/2643]
专题	海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang Zifeng,Xiao Tian,Pang Shaojun,et al. Isolation and identification of bacteria associated with the surfaces of several algal species[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):487-492.
APA	Wang Zifeng,Xiao Tian,Pang Shaojun,Liu Min,&Yue Haidong.(2009).Isolation and identification of bacteria associated with the surfaces of several algal species.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),487-492.
MLA	Wang Zifeng,et al."Isolation and identification of bacteria associated with the surfaces of several algal species".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):487-492.