A bioaugmentation failure caused by phage infection and weak biofilm formation ability

doi:10.1016/S1001-0742(08)62396-7

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	A bioaugmentation failure caused by phage infection and weak biofilm formation ability
	Fu Songzhe 1,2; Fan Hongxia 1,2; Liu Shuangjiang 1; Liu Ying3 ; Liu Zhipei 1
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
	2009
卷号	21 期号:8 页码:1153-1161
关键词	Marine Culture Water Circulation Ammonia Removal Lutimonas Sp H10 Nitrification Breakdown Fluorescent In Situ Hybridization (Fish)
ISSN号	1001-0742
DOI	10.1016/S1001-0742(08)62396-7
文献子类	Article
英文摘要	Two biological aerated filters (BAF) were setup for ammonia removal treatment of the circulation water in a marine aquaculture. One of the BAFs was bioaugmented with a heterotrophic nitrifying bacterium, Lutimonas sp. H10, where the ammonia removal was not improved and the massive inoculation was even followed by a nitrification breakdown from day 9 to 18. The nitrification was remained stable in control BAF operated under the same conditions. Fluorescent in situ hybridization (FISH) with rRNA-targeted probes and cultivable method revealed that Lutimonas sp. H10 almost disappeared from the bioaugomented BAF within 3 d, and this was mainly due to the infection of a specific phage as revealed by flask experiment, plaque assay and transmission electron observation. Analyses of 16S rRNA gene libraries showed that bacterial groups from two reactors evolved differently and an overgrowth of protozoa was observed in the bioaugmented BAR Therefore, phage infection and poor biofilm forming ability of the inoculated strain are the main reasons for bioaugmentation failure. In addition, gazing by protozoa of the bacteria might be the reason for the nitrification breakdown in bioaugmented BAF during day 9-18.; Two biological aerated filters (BAF) were setup for ammonia removal treatment of the circulation water in a marine aquaculture. One of the BAFs was bioaugmented with a heterotrophic nitrifying bacterium, Lutimonas sp. H10, where the ammonia removal was not improved and the massive inoculation was even followed by a nitrification breakdown from day 9 to 18. The nitrification was remained stable in control BAF operated under the same conditions. Fluorescent in situ hybridization (FISH) with rRNA-targeted probes and cultivable method revealed that Lutimonas sp. H10 almost disappeared from the bioaugomented BAF within 3 d, and this was mainly due to the infection of a specific phage as revealed by flask experiment, plaque assay and transmission electron observation. Analyses of 16S rRNA gene libraries showed that bacterial groups from two reactors evolved differently and an overgrowth of protozoa was observed in the bioaugmented BAR Therefore, phage infection and poor biofilm forming ability of the inoculated strain are the main reasons for bioaugmentation failure. In addition, gazing by protozoa of the bacteria might be the reason for the nitrification breakdown in bioaugmented BAF during day 9-18.
学科主题	Environmental Sciences
URL标识	查看原文
语种	英语
WOS记录号	WOS:000269139100020
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/2605]
专题	海洋研究所_海洋生态与环境科学重点实验室海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266701, Peoples R China
推荐引用方式 GB/T 7714	Fu Songzhe,Fan Hongxia,Liu Shuangjiang,et al. A bioaugmentation failure caused by phage infection and weak biofilm formation ability[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2009,21(8):1153-1161.
APA	Fu Songzhe,Fan Hongxia,Liu Shuangjiang,Liu Ying,&Liu Zhipei.(2009).A bioaugmentation failure caused by phage infection and weak biofilm formation ability.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,21(8),1153-1161.
MLA	Fu Songzhe,et al."A bioaugmentation failure caused by phage infection and weak biofilm formation ability".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 21.8(2009):1153-1161.