Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage

doi:10.1007/s00343-017-6109-2

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	Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage
	Xie Shijun1,2 ; Li Fuhua 1,3; Zhang Xiaojun 1; Zhang Jiquan 1; Xiang Jianhai1,3
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2017-11-01
卷号	35 期号:6 页码:1524-1530
关键词	Litopenaeus Vannamei Digestive Tract Peritrophin-like Protein Dsrna Interference Binding Specificity White Spot Syndrome Virus (Wssv) Reverse Gavage
DOI	10.1007/s00343-017-6109-2
文献子类	Article
英文摘要	The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus (WSSV) in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei (LvPT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigen (TM) design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at similar to 27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA (4 mu g per shrimp) was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection (hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.
语种	英语
WOS记录号	WOS:000415810900023
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/143280]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Xie Shijun,Li Fuhua,Zhang Xiaojun,et al. Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(6):1524-1530.
APA	Xie Shijun,Li Fuhua,Zhang Xiaojun,Zhang Jiquan,&Xiang Jianhai.(2017).Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(6),1524-1530.
MLA	Xie Shijun,et al."Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.6(2017):1524-1530.