Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48

doi:10.1007/s00343-017-6028-2

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48
	Xu Rui1,2 ; Li Xiaoming 1; Xu Gangming 1; Wang Bingui1
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2017-05-01
卷号	35 期号:3 页码:658-663
关键词	Aspergillus Wentii Asperolide a Anti-tumor Agent Fermentation Optimization
DOI	10.1007/s00343-017-6028-2
文献子类	Article
英文摘要	The marine algal-derived endophytic fungus Aspergillus wentii EN-48 produces the potential anti-tumor agent asperolide A, a tetranorlabdane diterpenoid active against lung cancer. However, the fermentation yield of asperolide A was very low and only produced in static cultures. Static fermentation conditions of A. wentii EN-48 were optimized employing response surface methodology to enhance the production of asperolide A. The optimized conditions resulted in a 13.9-fold yield enhancement, which matched the predicted value, and the optimized conditions were successfully used in scale-up fermentation for the production of asperolide A. Exogenous addition of plant hormones (especially 10 mu mol/L methyl jasmonate) stimulated asperolide A production. To our knowledge, this is first optimized production of an asperolide by a marine-derived fungus. The optimization is Effective and valuable to supply material for further anti-tumor mechanism studies and preclinical evaluation of asperolide A and other norditerpenoids.
语种	英语
WOS记录号	WOS:000399168400020
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/136720]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol,Lab Marine Biol & Biotec, Qingdao Natl Lab Marine Sci & Technol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu Rui,Li Xiaoming,Xu Gangming,et al. Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(3):658-663.
APA	Xu Rui,Li Xiaoming,Xu Gangming,&Wang Bingui.(2017).Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(3),658-663.
MLA	Xu Rui,et al."Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.3(2017):658-663.