Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus

doi:10.1016/j.fsi.2010.10.003

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	Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus
	Zhang, Min; Xiao, Zhi-zhong; Sun, Li
刊名	FISH & SHELLFISH IMMUNOLOGY
	2011
卷号	30 期号:1 页码:209-214
关键词	Tissue Factor Pathway Inhibitor 2 Sciaenops Ocellatus Bactericidal Activity
ISSN号	1050-4648
DOI	10.1016/j.fsi.2010.10.003
文献子类	Article
英文摘要	Tissue factor pathway inhibitor 2 (TFPI-2) is a structural homologue of TFPI, a potent inhibitor of tissue factor (TF)-mediated coagulation. Although TFPI-2 has been identified at sequence level in several fish species, no study on piscine TFPI-2 has been documented. In this report, we identified and analyzed a TFPI-2 homologue, SoTFPI2, from red drum Sciaenops ocellatus. The open reading frame of SoTFPI2 is 681 bp, which encodes a 226-residue protein that shares 59.2-82.3% overall sequence identities with known fish TFPI-2. SoTFPI2 possesses three tandem Kunitz domains and is negatively charged at the N-terminus and positively charged at the C-terminus. Expression of SoTFPI2 was detected, in increasing order, in spleen, muscle, gill, brain, liver, kidney, blood, and heart. Bacterial challenge and lipopolysaccharide treatment significantly upregulated SoTFPI2 expression in kidney in time-dependent manners. Recombinant SoTFPI2 purified from Escherichia colt inhibits the proteolytic activity of trypsin and exhibits bactericidal effect on a fish pathogen. Take together, these results indicate that SoTFPI2 is a biologically active serine protease inhibitor with antibacterial property and is likely to play a role in anti-bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.; Tissue factor pathway inhibitor 2 (TFPI-2) is a structural homologue of TFPI, a potent inhibitor of tissue factor (TF)-mediated coagulation. Although TFPI-2 has been identified at sequence level in several fish species, no study on piscine TFPI-2 has been documented. In this report, we identified and analyzed a TFPI-2 homologue, SoTFPI2, from red drum Sciaenops ocellatus. The open reading frame of SoTFPI2 is 681 bp, which encodes a 226-residue protein that shares 59.2-82.3% overall sequence identities with known fish TFPI-2. SoTFPI2 possesses three tandem Kunitz domains and is negatively charged at the N-terminus and positively charged at the C-terminus. Expression of SoTFPI2 was detected, in increasing order, in spleen, muscle, gill, brain, liver, kidney, blood, and heart. Bacterial challenge and lipopolysaccharide treatment significantly upregulated SoTFPI2 expression in kidney in time-dependent manners. Recombinant SoTFPI2 purified from Escherichia colt inhibits the proteolytic activity of trypsin and exhibits bactericidal effect on a fish pathogen. Take together, these results indicate that SoTFPI2 is a biologically active serine protease inhibitor with antibacterial property and is likely to play a role in anti-bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识	查看原文
语种	英语
WOS记录号	WOS:000286905800026
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11870]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Min,Xiao, Zhi-zhong,Sun, Li. Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):209-214.
APA	Zhang, Min,Xiao, Zhi-zhong,&Sun, Li.(2011).Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus.FISH & SHELLFISH IMMUNOLOGY,30(1),209-214.
MLA	Zhang, Min,et al."Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):209-214.