Characterization of flounder (Paralichthys olivaceus) FoxD3 and its function in regulating myogenic regulatory factors

doi:10.1007/s11626-011-9406-7

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	Characterization of flounder (Paralichthys olivaceus) FoxD3 and its function in regulating myogenic regulatory factors
	Zhang, Yuqing 1,2; Tan, Xungang1 ; Sun, Wei 1,2; Xu, Peng1,2 ; Zhang, Pei-Jun 1; Xu, Yongli 1
刊名	IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
	2011-06-01
卷号	47 期号:5-6 页码:399-405
关键词	Flounder Foxd3 Muscle Over-expression In Situ Hybridization Myogenic Regulatory Factors
ISSN号	1071-2690
DOI	10.1007/s11626-011-9406-7
文献子类	Article
英文摘要	As one member of winged helix domain transcription factors, FoxD3 plays an important role in the regulation of neural crest development and maintenance of mammalian stem cell lineages. A recent study showed that zebrafish FoxD3 is a downstream gene of Pax3 and can mediate the expression of Myf5. To further understand the function of FoxD3 in fish muscle development, we isolated the FoxD3 gene from flounder, and analyzed its expression pattern and function in regulating myogenic regulatory factors, MyoD and Myf5. In situ hybridization showed that flounder FoxD3 was firstly detected in the premigratory neural crest cells at 90% epiboly stage. The FoxD3 was expressed not only in neural crest cells but also in somite cells that will form muscle in the future. When flounder FoxD3 was over-expressed in zebrafish by microinjection, the expressions of zebrafish Myf5 and MyoD were both affected. It is possible that FoxD3 is involved in myogenesis by regulating the expression of Myf5 and MyoD. Also, over-expression of flounder FoxD3 in zebrafish inhibits the expression of zebrafish endogenic FoxD3.; As one member of winged helix domain transcription factors, FoxD3 plays an important role in the regulation of neural crest development and maintenance of mammalian stem cell lineages. A recent study showed that zebrafish FoxD3 is a downstream gene of Pax3 and can mediate the expression of Myf5. To further understand the function of FoxD3 in fish muscle development, we isolated the FoxD3 gene from flounder, and analyzed its expression pattern and function in regulating myogenic regulatory factors, MyoD and Myf5. In situ hybridization showed that flounder FoxD3 was firstly detected in the premigratory neural crest cells at 90% epiboly stage. The FoxD3 was expressed not only in neural crest cells but also in somite cells that will form muscle in the future. When flounder FoxD3 was over-expressed in zebrafish by microinjection, the expressions of zebrafish Myf5 and MyoD were both affected. It is possible that FoxD3 is involved in myogenesis by regulating the expression of Myf5 and MyoD. Also, over-expression of flounder FoxD3 in zebrafish inhibits the expression of zebrafish endogenic FoxD3.
学科主题	Cell Biology ; Developmental Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000291256200010
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11796]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋环流与波动重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yuqing,Tan, Xungang,Sun, Wei,et al. Characterization of flounder (Paralichthys olivaceus) FoxD3 and its function in regulating myogenic regulatory factors[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2011,47(5-6):399-405.
APA	Zhang, Yuqing,Tan, Xungang,Sun, Wei,Xu, Peng,Zhang, Pei-Jun,&Xu, Yongli.(2011).Characterization of flounder (Paralichthys olivaceus) FoxD3 and its function in regulating myogenic regulatory factors.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,47(5-6),399-405.
MLA	Zhang, Yuqing,et al."Characterization of flounder (Paralichthys olivaceus) FoxD3 and its function in regulating myogenic regulatory factors".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47.5-6(2011):399-405.