Characterization of amphioxus GDF8/11 gene, an archetype of vertebrate MSTN and GDF11

doi:10.1007/s00427-007-0162-3

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	Characterization of amphioxus GDF8/11 gene, an archetype of vertebrate MSTN and GDF11
	Xing, Fuguo ; Tan, Xungang; Zhang, Pei-Jun ; Ma, Junkai ; Zhang, Yuqing ; Xu, Peng; Xu, Yongli
刊名	DEVELOPMENT GENES AND EVOLUTION
	2007-07-01
卷号	217 期号:7 页码:549-554
关键词	Amphioxus Branchiostoma Belcheri Gdf8 Gdf11 Mstn Tgf-beta
ISSN号	0949-944X
DOI	10.1007/s00427-007-0162-3
文献子类	Article
英文摘要	MSTN, also known as growth and differentiation factor 8 (GDF8), and GDF11 are members of the transforming growth factor-beta (TGF-beta) subfamily. They have been thought to be derived from one ancestral gene. In the present study, we report the isolation and characterization of an invertebrate GDF8/11 homolog from the amphioxus (Branchiostoma belcheri tsingtauense). The amphioxus GDF8/11 gene consists of five exons flanked by four introns, which have two more exons and introns than that of other species. In intron III, a possible transposable element was identified. This suggested that this intron might be derived from transposon. The amphioxus GDF8/11 cDNA encodes a polypeptide of 419 amino acid residues. Phologenetic analysis shows that the GDF8/11 is at the base of vertebrate MSTNs and GDF11s. This result might prove that the GDF8/11 derived from one ancestral gene and the amphioxus GDF8/11 may be the common ancestral gene, and also the gene duplication event generating MSTN and GDF11 occurred before the divergence of vertebrates and after or at the divergence of amphioxus from vertebrates. Reverse transcriptase polymerase chain reaction results showed that the GDF8/11 gene was expressed in new fertilized cell, early gastrulation, and knife-shaped embryo, which was different from that in mammals. It suggested that the GDF8/11 gene might possess additional functions other than regulating muscle growth in amphioxus.; MSTN, also known as growth and differentiation factor 8 (GDF8), and GDF11 are members of the transforming growth factor-beta (TGF-beta) subfamily. They have been thought to be derived from one ancestral gene. In the present study, we report the isolation and characterization of an invertebrate GDF8/11 homolog from the amphioxus (Branchiostoma belcheri tsingtauense). The amphioxus GDF8/11 gene consists of five exons flanked by four introns, which have two more exons and introns than that of other species. In intron III, a possible transposable element was identified. This suggested that this intron might be derived from transposon. The amphioxus GDF8/11 cDNA encodes a polypeptide of 419 amino acid residues. Phologenetic analysis shows that the GDF8/11 is at the base of vertebrate MSTNs and GDF11s. This result might prove that the GDF8/11 derived from one ancestral gene and the amphioxus GDF8/11 may be the common ancestral gene, and also the gene duplication event generating MSTN and GDF11 occurred before the divergence of vertebrates and after or at the divergence of amphioxus from vertebrates. Reverse transcriptase polymerase chain reaction results showed that the GDF8/11 gene was expressed in new fertilized cell, early gastrulation, and knife-shaped embryo, which was different from that in mammals. It suggested that the GDF8/11 gene might possess additional functions other than regulating muscle growth in amphioxus.
学科主题	Cell Biology ; Evolutionary Biology ; Developmental Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000248169600006
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5691]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Xing, Fuguo,Tan, Xungang,Zhang, Pei-Jun,et al. Characterization of amphioxus GDF8/11 gene, an archetype of vertebrate MSTN and GDF11[J]. DEVELOPMENT GENES AND EVOLUTION,2007,217(7):549-554.
APA	Xing, Fuguo.,Tan, Xungang.,Zhang, Pei-Jun.,Ma, Junkai.,Zhang, Yuqing.,...&Xu, Yongli.(2007).Characterization of amphioxus GDF8/11 gene, an archetype of vertebrate MSTN and GDF11.DEVELOPMENT GENES AND EVOLUTION,217(7),549-554.
MLA	Xing, Fuguo,et al."Characterization of amphioxus GDF8/11 gene, an archetype of vertebrate MSTN and GDF11".DEVELOPMENT GENES AND EVOLUTION 217.7(2007):549-554.