Construction and characterization of a live, attenuated esrB mutant of Edwardsiella tarda and its potential as a vaccine against the haemorrhagic septicaemia in turbot, Scophthamus maximus (L.)

doi:10.1016/j.fsi.2006.11.002

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	Construction and characterization of a live, attenuated esrB mutant of Edwardsiella tarda and its potential as a vaccine against the haemorrhagic septicaemia in turbot, Scophthamus maximus (L.)
	Lan, Mo Zhao ; Peng, Xiao ; Xiang, Mao Yun ; Xia, Zou Yu ; Bo, Wang ; He, Li ; Li, Xu Yong ; Jun, Zhang Pei
刊名	FISH & SHELLFISH IMMUNOLOGY
	2007-09-01
卷号	23 期号:3 页码:521-530
关键词	Edwardsiella Tarda Esrb Mutant Live Attenuated Vaccine Turbot Scophthamus Maximus
ISSN号	1050-4648
DOI	10.1016/j.fsi.2006.11.002
文献子类	Article
英文摘要	The esrB gene of Edwardsiella tarda, which encodes a regulator protein of the type III secretion system, was mutated by the unmarked deletion method and reintroduced by allelic exchange into the chromosome of E. tarda LSE40 by means of the suicide vector pRE 112. The LSE40 esrB mutant was highly attenuated when inoculated intraperitoneally into turbot Scophthamus maximus L., showing a 50% lethal dose of 10(8.1) cfu/fish. The esrB mutants were not recoverable from the internal organs at 14 days post-inoculation. Vaccination with a single dose of 10(5)-10(7) cfu/fish of the esrB mutant elicited significant protection against the wildtype strain of E. tarda LSE40 (relative percentage survival > 50%). The protection correlated well with the antibody titres in the serum of vaccinated fish. (c) 2006 Elsevier Ltd. All rights reserved.; The esrB gene of Edwardsiella tarda, which encodes a regulator protein of the type III secretion system, was mutated by the unmarked deletion method and reintroduced by allelic exchange into the chromosome of E. tarda LSE40 by means of the suicide vector pRE 112. The LSE40 esrB mutant was highly attenuated when inoculated intraperitoneally into turbot Scophthamus maximus L., showing a 50% lethal dose of 10(8.1) cfu/fish. The esrB mutants were not recoverable from the internal organs at 14 days post-inoculation. Vaccination with a single dose of 10(5)-10(7) cfu/fish of the esrB mutant elicited significant protection against the wildtype strain of E. tarda LSE40 (relative percentage survival > 50%). The protection correlated well with the antibody titres in the serum of vaccinated fish. (c) 2006 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识	查看原文
语种	英语
WOS记录号	WOS:000248509800004
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5615]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean Univ China, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Lan, Mo Zhao,Peng, Xiao,Xiang, Mao Yun,et al. Construction and characterization of a live, attenuated esrB mutant of Edwardsiella tarda and its potential as a vaccine against the haemorrhagic septicaemia in turbot, Scophthamus maximus (L.)[J]. FISH & SHELLFISH IMMUNOLOGY,2007,23(3):521-530.
APA	Lan, Mo Zhao.,Peng, Xiao.,Xiang, Mao Yun.,Xia, Zou Yu.,Bo, Wang.,...&Jun, Zhang Pei.(2007).Construction and characterization of a live, attenuated esrB mutant of Edwardsiella tarda and its potential as a vaccine against the haemorrhagic septicaemia in turbot, Scophthamus maximus (L.).FISH & SHELLFISH IMMUNOLOGY,23(3),521-530.
MLA	Lan, Mo Zhao,et al."Construction and characterization of a live, attenuated esrB mutant of Edwardsiella tarda and its potential as a vaccine against the haemorrhagic septicaemia in turbot, Scophthamus maximus (L.)".FISH & SHELLFISH IMMUNOLOGY 23.3(2007):521-530.