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	White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis
	Wang, YT; Liu, W; Seah, JN; Lam, CS; Xiang, JH; Korzh, V; Kwang, J
刊名	DISEASES OF AQUATIC ORGANISMS
	2002-12-10
卷号	52期号:3页码:249-259
关键词	White Spot Syndrome Virus Wssv Penaeus Merguiensis Hemocyte Flow Cytometry Immunofluorescence Assay Ifa
ISSN号	0177-5103
文献子类	Article
英文摘要	White spot syndrome virus (WSSV) was specifically detected by PCR in Penaeus merguiensis hemocytes, hemolymph and plasma. This suggested a close association between the shrimp hemolymph and the virus. Three types of hemocyte from shrimp were isolated using flow cytometry. Dynamic changes of the hemocyte subpopulations in P. merguiensis at different times after infection were observed, indicating that the WSSV infection selectively affected specific subpopulations. Immunofluorescence assay (IFA) and a Wright-Giemsa double staining study of hemocyte types further confirmed the cellular localization of the virus in the infected hemocytes. Electron microscopy revealed virus particles in both vacuoles and the nucleus of the semigranular cells (SGC), as well as in the vacuoles of the granular cells (GC). However, no virus could be detected in the hyaline cells (HC). Our results suggest that the virus infects 2 types of shrimp hemocytes-GCs and SGCs. The SGC type contains higher virus loads and exhibits faster infection rates, and is apparently more susceptible to WSSV infection.; White spot syndrome virus (WSSV) was specifically detected by PCR in Penaeus merguiensis hemocytes, hemolymph and plasma. This suggested a close association between the shrimp hemolymph and the virus. Three types of hemocyte from shrimp were isolated using flow cytometry. Dynamic changes of the hemocyte subpopulations in P. merguiensis at different times after infection were observed, indicating that the WSSV infection selectively affected specific subpopulations. Immunofluorescence assay (IFA) and a Wright-Giemsa double staining study of hemocyte types further confirmed the cellular localization of the virus in the infected hemocytes. Electron microscopy revealed virus particles in both vacuoles and the nucleus of the semigranular cells (SGC), as well as in the vacuoles of the granular cells (GC). However, no virus could be detected in the hyaline cells (HC). Our results suggest that the virus infects 2 types of shrimp hemocytes-GCs and SGCs. The SGC type contains higher virus loads and exhibits faster infection rates, and is apparently more susceptible to WSSV infection.
学科主题	Fisheries ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000180975400008
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3297]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Natl Univ Singapore, Inst Mol Agrobiol, Singapore 117604, Singapore 2.Chinese Acad Sci, Inst Oceanol, Shandong 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, YT,Liu, W,Seah, JN,et al. White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis[J]. DISEASES OF AQUATIC ORGANISMS,2002,52(3):249-259.
APA	Wang, YT.,Liu, W.,Seah, JN.,Lam, CS.,Xiang, JH.,...&Kwang, J.(2002).White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis.DISEASES OF AQUATIC ORGANISMS,52(3),249-259.
MLA	Wang, YT,et al."White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis".DISEASES OF AQUATIC ORGANISMS 52.3(2002):249-259.

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