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	Improvement of polyploidy induction in Eriocheir sinensis
	Cui, ZX; Xiang, JH; Zhou, LH; Cai, NN; Song, LS
刊名	ACTA OCEANOLOGICA SINICA
	2004
卷号	23期号:4页码:725-732
关键词	Eriocheir Sinensis Triploid Tetraploid Zoea
ISSN号	0253-505X
文献子类	Article
英文摘要	An incubating temperature of 15 degreesC is used to induce triploidy in Etiocheir sinensis through inhibition of the release of polar body H, and that of 18 degreesC to induce tetraploidy through inhibition of the first cleavage. Flow cytometry is used to identify the ploidy in different developmental stages. For induction of triploidy in fertilized eggs in vitro, the highest induction rate observed in blastula by cytochalasin B, 6-DMAP and KCI is 49.1%, 51.7% and 77.5%, respectively. In the KCI treatment of pregnant crabs with the fertilized eggs, the highest triploid induction rate observed in the zoea is 85.3%. For induction of tetraploidy, the highest induction rate observed in the blastula by cytochaslasin 13, 6-DMAP and KCI is 50.3%, 54.9% and 79.8% respectively. In the KCI treatment of pregnant crabs with the fertilized eggs, the highest induction rate in zoea is 27.3%. Through this study such difficulty as in vitro culture is overcome. Triploid zoea Etiocheir sinensis has been developed for the first time. The induction rate of tetraploid zoea has also been greatly improved.; An incubating temperature of 15 degreesC is used to induce triploidy in Etiocheir sinensis through inhibition of the release of polar body H, and that of 18 degreesC to induce tetraploidy through inhibition of the first cleavage. Flow cytometry is used to identify the ploidy in different developmental stages. For induction of triploidy in fertilized eggs in vitro, the highest induction rate observed in blastula by cytochalasin B, 6-DMAP and KCI is 49.1%, 51.7% and 77.5%, respectively. In the KCI treatment of pregnant crabs with the fertilized eggs, the highest triploid induction rate observed in the zoea is 85.3%. For induction of tetraploidy, the highest induction rate observed in the blastula by cytochaslasin 13, 6-DMAP and KCI is 50.3%, 54.9% and 79.8% respectively. In the KCI treatment of pregnant crabs with the fertilized eggs, the highest induction rate in zoea is 27.3%. Through this study such difficulty as in vitro culture is overcome. Triploid zoea Etiocheir sinensis has been developed for the first time. The induction rate of tetraploid zoea has also been greatly improved.
学科主题	Oceanography
语种	英语
WOS记录号	WOS:000226085700016
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3187]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, EMBL, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Cui, ZX,Xiang, JH,Zhou, LH,et al. Improvement of polyploidy induction in Eriocheir sinensis[J]. ACTA OCEANOLOGICA SINICA,2004,23(4):725-732.
APA	Cui, ZX,Xiang, JH,Zhou, LH,Cai, NN,&Song, LS.(2004).Improvement of polyploidy induction in Eriocheir sinensis.ACTA OCEANOLOGICA SINICA,23(4),725-732.
MLA	Cui, ZX,et al."Improvement of polyploidy induction in Eriocheir sinensis".ACTA OCEANOLOGICA SINICA 23.4(2004):725-732.

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