Polymorphic EST-SSR markers and their mode of inheritance in Fenneropenaeus chinensis

doi:10.1016/j.aquaculture.2005.03.041

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	Polymorphic EST-SSR markers and their mode of inheritance in Fenneropenaeus chinensis
	Wang, HX; Li, FH; Xiang, HH
刊名	AQUACULTURE
	2005-09-12
卷号	249 期号:1-4 页码:107-114
关键词	Chinese Shrimp Fenneropenaeus Chinensis Est-ssr Microsatellite
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2005.03.041
文献子类	Article
英文摘要	229 SSRs (simple sequence repeats) were identified among 10,443 ESTs (expressed sequence tags) of Chinese shrimp (Fenneropenaeus chinensis). The average density of SSRs was one SSR per 19.1 kb of EST sequence screened. The dinucleotide repeats appeared to be the most abundant SSRs detected. Nine EST-SSR markers were detected polymorphisms of the thirty SSR primer pairs derived from F chinensis ESTs. The number of alleles per locus ranged from 5 to 15, with an average of 9.1 alleles per locus. The observed heterozygosity of nine loci ranged from 0.47 to 0.87. These loci were used successfully for pedigree analysis in three families of Fenneropenaeus chinensis. Two of the nine microsatellite loci showed the existence of null alleles. Assuming the existence of null alleles at Fc07 and Fc14 loci, the allelic inheritance mode of the EST-SSR DNA markers (Fc04, Fc06, Fc07, Fc10, Fc14, Fc18, Fc22, Fc24, and Fc27) was consistent with Mendelian segregation. (c) 2005 Elsevier B.V. All rights reserved.; 229 SSRs (simple sequence repeats) were identified among 10,443 ESTs (expressed sequence tags) of Chinese shrimp (Fenneropenaeus chinensis). The average density of SSRs was one SSR per 19.1 kb of EST sequence screened. The dinucleotide repeats appeared to be the most abundant SSRs detected. Nine EST-SSR markers were detected polymorphisms of the thirty SSR primer pairs derived from F chinensis ESTs. The number of alleles per locus ranged from 5 to 15, with an average of 9.1 alleles per locus. The observed heterozygosity of nine loci ranged from 0.47 to 0.87. These loci were used successfully for pedigree analysis in three families of Fenneropenaeus chinensis. Two of the nine microsatellite loci showed the existence of null alleles. Assuming the existence of null alleles at Fc07 and Fc14 loci, the allelic inheritance mode of the EST-SSR DNA markers (Fc04, Fc06, Fc07, Fc10, Fc14, Fc18, Fc22, Fc24, and Fc27) was consistent with Mendelian segregation. (c) 2005 Elsevier B.V. All rights reserved.
学科主题	Fisheries ; Marine & Freshwater Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000231765000012
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3141]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, HX,Li, FH,Xiang, HH. Polymorphic EST-SSR markers and their mode of inheritance in Fenneropenaeus chinensis[J]. AQUACULTURE,2005,249(1-4):107-114.
APA	Wang, HX,Li, FH,&Xiang, HH.(2005).Polymorphic EST-SSR markers and their mode of inheritance in Fenneropenaeus chinensis.AQUACULTURE,249(1-4),107-114.
MLA	Wang, HX,et al."Polymorphic EST-SSR markers and their mode of inheritance in Fenneropenaeus chinensis".AQUACULTURE 249.1-4(2005):107-114.